Molecular identification of an endemic Alpine mammal, Apodemus alpicola, using a PCR-based RFLP method

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Serval ID
serval:BIB_D17DA8152E4E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular identification of an endemic Alpine mammal, Apodemus alpicola, using a PCR-based RFLP method
Journal
Revue Suisse de Zoologie
Author(s)
Reutter B. A., Petit E., Vogel P.
ISSN
0035-418X
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
109
Number
1
Pages
9-16
Language
english
Abstract
The ability of a PCR-based restriction fragment length polymorphism (RFLP) analysis of the cytochrome b (mtDNA) to distinguish Apodemus alpicola from two other Apodemus species was investigated. The partial sequencing of the cytochrome b allowed the identification of one enzyme as being potentially diagnostic. This was supported by an analysis of 131 specimens previously identified using morphometric and/or allozymic data, indicating that the PCR-based RFLP method provides a rapid and reliable tool for distinguishing A. alpicola from its two co-occurring congenerics. The method is applicable to samples taken in the field for ecological studies, and could easily be adapted to the identification of museum samples.
Keywords
apodemus mtdna pcr rflp identification mitochondrial-DNA sylvaticus flavicollis populations divergence rodentia
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Create date
24/01/2008 17:33
Last modification date
20/08/2019 15:51
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