Leishmania metacaspase: an arginine-specific peptidase.

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State: Public
Version: author
Serval ID
serval:BIB_B3F360C931E2
Type
Article: article from journal or magazin.
Collection
Publications
Title
Leishmania metacaspase: an arginine-specific peptidase.
Journal
Methods in Molecular Biology
Author(s)
Martin R., Gonzalez I., Fasel N.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
1133
Pages
189-202
Language
english
Abstract
The purpose of this chapter is to give insights into metacaspase of Leishmania protozoan parasites as arginine-specific cysteine peptidase. The physiological role of metacaspase in Leishmania is still a matter of debate, whereas its peptidase enzymatic activity has been well characterized. Among the different possible expression systems, metacaspase-deficient yeast cells (Δyca1) have been instrumental in studying the activity of Leishmania major metacaspase (LmjMCA). Here, we describe techniques for purification of LmjMCA and its activity measurement, providing a platform for further identification of LmjMCA substrates.
Keywords
Leishmania, Cysteine peptidase, Arginine-specific peptidase, Metacaspase, Enzymatic assay, Protease inhibitors
Pubmed
Web of science
Open Access
Yes
Create date
26/06/2014 9:59
Last modification date
20/08/2019 16:22
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