The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes.

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State: Public
Version: Final published version
Serval ID
serval:BIB_9E64C3251560
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes.
Journal
Journal of Cell Science
Author(s)
Yang H.Y., Charles R.P., Hummler E., Baines D.L., Isseroff R.R.
ISSN
1477-9137 (Electronic)
ISSN-L
0021-9533
Publication state
Published
Issued date
2013
Volume
126
Number
Pt 9
Pages
1942-1951
Language
english
Notes
Publication types: Journal ArticlePublication Status: ppublish. PDF type: Research article
Abstract
Cellular directional migration in an electric field (galvanotaxis) is one of the mechanisms guiding cell movement in embryogenesis and in skin epidermal repair. The epithelial sodium channel (ENaC), in addition to its function of regulating sodium transport in kidney, has recently been found to modulate cell locomotory speed. Here we tested whether ENaC has an additional function of mediating the directional migration of galvanotaxis in keratinocytes. Genetic depletion of ENaC completely blocks only galvanotaxis and does not decrease migration speed. Overexpression of ENaC is sufficient to drive galvanotaxis in otherwise unresponsive cells. Pharmacologic blockade or maintenance of the open state of ENaC also decreases or increases, respectively, galvanotaxis, suggesting that the channel open state is responsible for the response. Stable lamellipodial extensions formed at the cathodal sides of wild-type cells at the start of galvanotaxis; these were absent in the ENaC knockout keratinocytes, suggesting that ENaC mediates galvanotaxis by generating stable lamellipodia that steer cell migration. We provide evidence that ENaC is required for directional migration of keratinocytes in an electric field, supporting a role for ENaC in skin wound healing.
Pubmed
Web of science
Open Access
Yes
Create date
04/07/2013 19:50
Last modification date
20/10/2020 10:08
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