Investigation of the global protein content from healthy human tears.

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Serval ID
serval:BIB_74008C08D4B0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Investigation of the global protein content from healthy human tears.
Journal
Experimental eye research
Author(s)
Dor M., Eperon S., Lalive P.H., Guex-Crosier Y., Hamedani M., Salvisberg C., Turck N.
ISSN
1096-0007 (Electronic)
ISSN-L
0014-4835
Publication state
Published
Issued date
02/2019
Peer-reviewed
Oui
Volume
179
Pages
64-74
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Considering absence of invasiveness and side effects, tears emerge as a particularly attractive fluid for biomarker discovery and therefore for daily clinical use. However, to date this fluid remains poorly studied in healthy condition. Here, we present an updated in-depth characterisation of the human healthy tear protein composition using proteomics approach. Both eyes of 8 healthy controls (4 men and 4 women, average age: 38 ± 18) were collected using the Schirmer's strip method. After liquid digestion and off-gel electrophoresis fractionation, three independent proteomics analyses were performed on a LTQ-Orbitrap Velos Pro. Globally, 1351 proteins were identified with 2 unique peptides and 1% FDR. Gene ontology analyses showed up that 39% of the tear proteins were enzymes, with high numbers of dehydrogenases, phosphatases, kinases and ligases. Immunoglobulins, serpins and 14-3-3 domains proteins also emerged as the main tear protein families. The glycolysis and the coagulation and complement cascades, which were already shown in tears as involved in ocular and systemic diseases, were highlighted performing pathway analyses. Our study therefore complements the existing data on healthy tears proteome. Nevertheless, extensive studies for deeply and definitively characterise this promising fluid are required in the near future in order to be able to routinely use this fluid in clinics. A better understanding of its protein content will probably open new avenues in the biomarker discovery and clinical practice in the near future.
Keywords
Adult, Biomarkers/metabolism, Electrophoresis, Polyacrylamide Gel, Eye Proteins/metabolism, Female, Healthy Volunteers, Humans, Male, Mass Spectrometry, Middle Aged, Proteomics, Tears/metabolism, Young Adult, Gas-phase fractionation, Integrative pathways, Off-gel electrophoresis, Proteome, Tears
Pubmed
Web of science
Create date
30/10/2018 12:38
Last modification date
20/08/2019 14:31
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