Natural alleles at the <i>Doa</i> locus underpin evolutionary changes in <i>Drosophila</i> lifespan and fecundity.

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License: CC BY 4.0
Serval ID
serval:BIB_6B05F1D08FA1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Natural alleles at the <i>Doa</i> locus underpin evolutionary changes in <i>Drosophila</i> lifespan and fecundity.
Journal
Proceedings. Biological sciences
Author(s)
Hoedjes K.M., Kostic H., Keller L., Flatt T.
ISSN
1471-2954 (Electronic)
ISSN-L
0962-8452
Publication state
Published
Issued date
09/11/2022
Peer-reviewed
Oui
Volume
289
Number
1986
Pages
20221989
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
'Evolve and resequence' (E&amp;R) studies in <i>Drosophila melanogaster</i> have identified many candidate loci underlying the evolution of ageing and life history, but experiments that validate the effects of such candidates remain rare. In a recent E&amp;R study we have identified several alleles of the LAMMER kinase <i>Darkener of apricot</i> (<i>Doa</i>) as candidates for evolutionary changes in lifespan and fecundity. Here, we use two complementary approaches to confirm a functional role of <i>Doa</i> in life-history evolution. First, we used transgenic RNAi to study the effects of <i>Doa</i> at the whole-gene level. Ubiquitous silencing of expression in adult flies reduced both lifespan and fecundity, indicating pleiotropic effects. Second, to characterize segregating variation at <i>Doa</i>, we examined four candidate single nucleotide polymorphisms (SNPs; <i>Doa-1</i>, -<i>2</i>, -<i>3</i>, <i>-4</i>) using a genetic association approach. Three candidate SNPs had effects that were qualitatively consistent with expectations based on our E&amp;R study: <i>Doa-2</i> pleiotropically affected both lifespan and late-life fecundity; <i>Doa-1</i> affected lifespan (but not fecundity); and <i>Doa-4</i> affected late-life fecundity (but not lifespan). Finally, the last candidate allele (<i>Doa-3</i>) also affected lifespan, but in the opposite direction from predicted.
Keywords
Animals, Drosophila melanogaster/genetics, Drosophila melanogaster/metabolism, Drosophila/genetics, Alleles, Prunus armeniaca/genetics, Prunus armeniaca/metabolism, Longevity, Drosophila Proteins/genetics, Drosophila Proteins/metabolism, Drosophila, ageing, experimental evolution, pleiotropy, reproduction
Pubmed
Open Access
Yes
Create date
16/11/2022 16:03
Last modification date
17/11/2022 6:42
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