RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae.

Details

Serval ID
serval:BIB_66E1D434E25B
Type
Article: article from journal or magazin.
Collection
Publications
Title
RocS drives chromosome segregation and nucleoid protection in Streptococcus pneumoniae.
Journal
Nature microbiology
Author(s)
Mercy C., Ducret A., Slager J., Lavergne J.P., Freton C., Nagarajan S.N., Garcia P.S., Noirot-Gros M.F., Dubarry N., Nourikyan J., Veening J.W., Grangeasse C.
ISSN
2058-5276 (Electronic)
ISSN-L
2058-5276
Publication state
Published
Issued date
10/2019
Peer-reviewed
Oui
Volume
4
Number
10
Pages
1661-1670
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Chromosome segregation in bacteria is poorly understood outside some prominent model strains <sup>1-5</sup> and even less is known about how it is coordinated with other cellular processes. This is the case for the opportunistic human pathogen Streptococcus pneumoniae (the pneumococcus) <sup>6</sup> , which lacks the Min and the nucleoid occlusion systems <sup>7</sup> , and possesses only an incomplete chromosome partitioning Par(A)BS system, in which ParA is absent <sup>8</sup> . The bacterial tyrosine kinase <sup>9</sup> CpsD, which is required for capsule production, was previously found to interfere with chromosome segregation <sup>10</sup> . Here, we identify a protein of unknown function that interacts with CpsD and drives chromosome segregation. RocS (Regulator of Chromosome Segregation) is a membrane-bound protein that interacts with both DNA and the chromosome partitioning protein ParB to properly segregate the origin of replication region to new daughter cells. In addition, we show that RocS interacts with the cell division protein FtsZ and hinders cell division. Altogether, this work reveals that RocS is the cornerstone of a nucleoid protection system ensuring proper chromosome segregation and cell division in coordination with the biogenesis of the protective capsular layer.
Keywords
Bacterial Capsules/metabolism, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Cell Division, Chromosome Segregation, Cytoskeletal Proteins/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Gene Deletion, Models, Biological, Origin Recognition Complex/genetics, Origin Recognition Complex/metabolism, Protein-Tyrosine Kinases/genetics, Protein-Tyrosine Kinases/metabolism, Streptococcus pneumoniae/cytology, Streptococcus pneumoniae/genetics, Streptococcus pneumoniae/metabolism
Pubmed
Web of science
Create date
24/06/2019 8:18
Last modification date
24/01/2020 7:19
Usage data