Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon

Details

Serval ID
serval:BIB_5F8CC9DC3DF6
Type
Article: article from journal or magazin.
Collection
Publications
Title
Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon
Journal
Infect Immun
Author(s)
Dacheux D., Epaulard O., de Groot A., Guery B., Leberre R., Attree I., Polack B., Toussaint B.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Publication state
Published
Issued date
07/2002
Volume
70
Number
7
Pages
3973-7
Language
english
Notes
Dacheux, Denis
Epaulard, Olivier
de Groot, Arjan
Guery, Benoit
Leberre, Rozen
Attree, Ina
Polack, Benoit
Toussaint, Bertrand
eng
Research Support, Non-U.S. Gov't
Infect Immun. 2002 Jul;70(7):3973-7. doi: 10.1128/iai.70.7.3973-3977.2002.
Abstract
Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.
Keywords
Animals, Bacterial Proteins/metabolism, Cystic Fibrosis/microbiology, DNA-Binding Proteins/genetics/physiology, Dihydrolipoyllysine-Residue Acetyltransferase, Disease Models, Animal, Histidine Kinase, Humans, L-Lactate Dehydrogenase/metabolism, Mutagenesis, Insertional, Neutrophils/microbiology, Operon/*physiology, Phosphoprotein Phosphatases/genetics/metabolism, Pneumonia, Bacterial/microbiology, Pore Forming Cytotoxic Proteins, Protein Kinases/metabolism, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/*enzymology/genetics/isolation &, purification/pathogenicity, Pyruvate Dehydrogenase Complex/genetics/*physiology, Rats, Rats, Sprague-Dawley, Trans-Activators/genetics/physiology
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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