Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.

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Serval ID
serval:BIB_5F8CC9DC3DF6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Production externe
Title
Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.
Journal
Infection and immunity
Author(s)
Dacheux D., Epaulard O., de Groot A., Guery B., Leberre R., Attree I., Polack B., Toussaint B.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Publication state
Published
Issued date
07/2002
Peer-reviewed
Oui
Volume
70
Number
7
Pages
3973-3977
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.
Keywords
Animals, Bacterial Proteins/metabolism, Cystic Fibrosis/microbiology, DNA-Binding Proteins/genetics, DNA-Binding Proteins/physiology, Dihydrolipoyllysine-Residue Acetyltransferase, Disease Models, Animal, Histidine Kinase, Humans, L-Lactate Dehydrogenase/metabolism, Mutagenesis, Insertional, Neutrophils/microbiology, Operon/physiology, Phosphoprotein Phosphatases/genetics, Phosphoprotein Phosphatases/metabolism, Pneumonia, Bacterial/microbiology, Pore Forming Cytotoxic Proteins, Protein Kinases/metabolism, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/isolation & purification, Pseudomonas aeruginosa/pathogenicity, Pyruvate Dehydrogenase Complex/genetics, Pyruvate Dehydrogenase Complex/physiology, Rats, Rats, Sprague-Dawley, Trans-Activators/genetics, Trans-Activators/physiology
DOI
10.1128/IAI.70.7.3973-3977.2002
Pubmed
12065547
Web of science
000176302600081
Open Access
Yes
Create date
29/04/2021 10:59
Last modification date
17/07/2023 10:58
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