Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.

Détails

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ID Serval
serval:BIB_5F8CC9DC3DF6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Production externe
Titre
Activation of the Pseudomonas aeruginosa type III secretion system requires an intact pyruvate dehydrogenase aceAB operon.
Périodique
Infection and immunity
Auteur⸱e⸱s
Dacheux D., Epaulard O., de Groot A., Guery B., Leberre R., Attree I., Polack B., Toussaint B.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Statut éditorial
Publié
Date de publication
07/2002
Peer-reviewed
Oui
Volume
70
Numéro
7
Pages
3973-3977
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Pseudomonas aeruginosa clinical cystic fibrosis isolate CHA was mutagenized with Tn5Tc to identify new genes involved in type III secretion system (TTSS)-dependent cytotoxicity toward human polymorphonuclear neutrophils. Among 25 mutants affected in TTSS function, 14 contained the insertion at different positions in the aceAB operon encoding the PDH-E1 and -E2 subunits of pyruvate dehydrogenase. In PDH mutants, no transcriptional activation of TTSS genes in response to calcium depletion occurred. Expression in trans of ExsA restored TTSS function and cytotoxicity.
Mots-clé
Animals, Bacterial Proteins/metabolism, Cystic Fibrosis/microbiology, DNA-Binding Proteins/genetics, DNA-Binding Proteins/physiology, Dihydrolipoyllysine-Residue Acetyltransferase, Disease Models, Animal, Histidine Kinase, Humans, L-Lactate Dehydrogenase/metabolism, Mutagenesis, Insertional, Neutrophils/microbiology, Operon/physiology, Phosphoprotein Phosphatases/genetics, Phosphoprotein Phosphatases/metabolism, Pneumonia, Bacterial/microbiology, Pore Forming Cytotoxic Proteins, Protein Kinases/metabolism, Pseudomonas Infections/microbiology, Pseudomonas aeruginosa/enzymology, Pseudomonas aeruginosa/genetics, Pseudomonas aeruginosa/isolation & purification, Pseudomonas aeruginosa/pathogenicity, Pyruvate Dehydrogenase Complex/genetics, Pyruvate Dehydrogenase Complex/physiology, Rats, Rats, Sprague-Dawley, Trans-Activators/genetics, Trans-Activators/physiology
DOI
10.1128/IAI.70.7.3973-3977.2002
Pubmed
12065547
Web of science
000176302600081
Open Access
Oui
Création de la notice
29/04/2021 10:59
Dernière modification de la notice
17/07/2023 10:58
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