Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport
Details
Serval ID
serval:BIB_5C8B7A17DA2B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport
Journal
FEBS Letters
ISSN
0014-5793 (Print)
Publication state
Published
Issued date
08/1990
Volume
269
Number
1
Pages
105-8
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 20
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 20
Abstract
In this study, we have followed the fate of newly synthesized alpha- and beta-subunits of Na,K-ATPase in Xenopus oocytes injected with alpha and/or beta cRNA to examine whether assembly of the two subunits is needed for a correct folding and/or for intracellular transport of Na,K-ATPase. Our data indicate that (1) assembly of alpha- and beta-subunits occurs at the level of the ER, (2) beta-subunits are needed for the newly synthesized alpha-subunit to adopt a stable configuration and (3) alpha- and beta-subunits mutually depend on each other to be transported out of the ER.
Keywords
Animals
Biological Transport
Cell Compartmentation
Endoplasmic Reticulum/metabolism
Glycosylation
Macromolecular Substances
Membrane Glycoproteins/*metabolism/ultrastructure
Metabolic Clearance Rate
Microinjections
Na(+)-K(+)-Exchanging ATPase/genetics/*metabolism
Precipitin Tests
Protein Conformation
Protein Processing, Post-Translational
RNA, Messenger/genetics
Time Factors
Xenopus laevis
Pubmed
Web of science
Create date
24/01/2008 13:28
Last modification date
20/08/2019 15:15