Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport

Details

Serval ID
serval:BIB_5C8B7A17DA2B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Mutual dependence of Na,K-ATPase alpha- and beta-subunits for correct posttranslational processing and intracellular transport
Journal
FEBS Letters
Author(s)
Ackermann  U., Geering  K.
ISSN
0014-5793 (Print)
Publication state
Published
Issued date
08/1990
Volume
269
Number
1
Pages
105-8
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 20
Abstract
In this study, we have followed the fate of newly synthesized alpha- and beta-subunits of Na,K-ATPase in Xenopus oocytes injected with alpha and/or beta cRNA to examine whether assembly of the two subunits is needed for a correct folding and/or for intracellular transport of Na,K-ATPase. Our data indicate that (1) assembly of alpha- and beta-subunits occurs at the level of the ER, (2) beta-subunits are needed for the newly synthesized alpha-subunit to adopt a stable configuration and (3) alpha- and beta-subunits mutually depend on each other to be transported out of the ER.
Keywords
Animals Biological Transport Cell Compartmentation Endoplasmic Reticulum/metabolism Glycosylation Macromolecular Substances Membrane Glycoproteins/*metabolism/ultrastructure Metabolic Clearance Rate Microinjections Na(+)-K(+)-Exchanging ATPase/genetics/*metabolism Precipitin Tests Protein Conformation Protein Processing, Post-Translational RNA, Messenger/genetics Time Factors Xenopus laevis
Pubmed
Web of science
Create date
24/01/2008 12:28
Last modification date
20/08/2019 14:15
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