A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane.

Details

Ressource 1Download: BIB_523F78C4557D.P001.pdf (4000.99 [Ko])
State: Public
Version: Final published version
Serval ID
serval:BIB_523F78C4557D
Type
Article: article from journal or magazin.
Collection
Publications
Title
A phosphorylation cycle shapes gradients of the DYRK family kinase Pom1 at the plasma membrane.
Journal
Cell
Author(s)
Hachet O., Berthelot-Grosjean M., Kokkoris K., Vincenzetti V., Moosbrugger J., Martin S.G.
ISSN
1097-4172 (Electronic)
ISSN-L
0092-8674
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
145
Number
7
Pages
1116-1128
Language
english
Abstract
Concentration gradients regulate many cell biological and developmental processes. In rod-shaped fission yeast cells, polar cortical gradients of the DYRK family kinase Pom1 couple cell length with mitotic commitment by inhibiting a mitotic inducer positioned at midcell. However, how Pom1 gradients are established is unknown. Here, we show that Tea4, which is normally deposited at cell tips by microtubules, is both necessary and, upon ectopic cortical localization, sufficient to recruit Pom1 to the cell cortex. Pom1 then moves laterally at the plasma membrane, which it binds through a basic region exhibiting direct lipid interaction. Pom1 autophosphorylates in this region to lower lipid affinity and promote membrane release. Tea4 triggers Pom1 plasma membrane association by promoting its dephosphorylation through the protein phosphatase 1 Dis2. We propose that local dephosphorylation induces Pom1 membrane association and nucleates a gradient shaped by the opposing actions of lateral diffusion and autophosphorylation-dependent membrane detachment.
Keywords
Amino Acid Sequence, Cell Cycle, Cell Membrane/metabolism, Microtubule-Associated Proteins/metabolism, Molecular Sequence Data, Phosphorylation, Protein Kinases/chemistry, Protein Kinases/metabolism, Schizosaccharomyces/cytology, Schizosaccharomyces/metabolism, Schizosaccharomyces pombe Proteins/metabolism, Sequence Alignment
Pubmed
Web of science
Open Access
Yes
Create date
12/10/2011 12:46
Last modification date
20/08/2019 14:07
Usage data