Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion.

Details

Ressource 1Download: Supplemental_Data.pdf (2144.62 [Ko])
State: Public
Version: Final published version
Secondary document(s)
Download: Supplemental_Data.pdf (4277.74 [Ko])
State: Public
Version: Supplementary document
Serval ID
serval:BIB_0017F15B0B98
Type
Article: article from journal or magazin.
Collection
Publications
Title
Spatial focalization of pheromone/MAPK signaling triggers commitment to cell-cell fusion.
Journal
Genes and Development
Author(s)
Dudin O., Merlini L., Martin S.G.
ISSN
0890-9369 (Print)
ISSN-L
0890-9369
Publication state
Published
Issued date
2016
Peer-reviewed
Oui
Volume
30
Number
19
Pages
2226-2239
Language
english
Abstract
Cell fusion is universal in eukaryotes for fertilization and development, but what signals this process is unknown. Here, we show in Schizosaccharomyces pombe that fusion does not require a dedicated signal but is triggered by spatial focalization of the same pheromone-GPCR (G-protein-coupled receptor)-MAPK signaling cascade that drives earlier mating events. Autocrine cells expressing the receptor for their own pheromone trigger fusion attempts independently of cell-cell contact by concentrating pheromone release at the fusion focus, a dynamic actin aster underlying the secretion of cell wall hydrolases. Pheromone receptor and MAPK cascade are similarly enriched at the fusion focus, concomitant with fusion commitment in wild-type mating pairs. This focalization promotes cell fusion by immobilizing the fusion focus, thus driving local cell wall dissolution. We propose that fusion commitment is imposed by a local increase in MAPK concentration at the fusion focus, driven by a positive feedback between fusion focus formation and focalization of pheromone release and perception.

Keywords
G-protein-coupled receptor signaling, MAP kinase cascade, cell–cell fusion, fertilization, fission yeast Schizosaccharomyces pombe, formin, pheromone
Pubmed
Web of science
Open Access
Yes
Create date
28/11/2016 16:06
Last modification date
20/08/2019 13:22
Usage data