Circadian and feeding rhythms differentially affect rhythmic mRNA transcription and translation in mouse liver.

Détails

ID Serval
serval:BIB_FFCC0CC29FFF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Circadian and feeding rhythms differentially affect rhythmic mRNA transcription and translation in mouse liver.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Atger F., Gobet C., Marquis J., Martin E., Wang J., Weger B., Lefebvre G., Descombes P., Naef F., Gachon F.
ISSN
1091-6490 (Electronic)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
112
Numéro
47
Pages
E6579-E6588
Langue
anglais
Résumé
Diurnal oscillations of gene expression are a hallmark of rhythmic physiology across most living organisms. Such oscillations are controlled by the interplay between the circadian clock and feeding rhythms. Although rhythmic mRNA accumulation has been extensively studied, comparatively less is known about their transcription and translation. Here, we quantified simultaneously temporal transcription, accumulation, and translation of mouse liver mRNAs under physiological light-dark conditions and ad libitum or night-restricted feeding in WT and brain and muscle Arnt-like 1 (Bmal1)-deficient animals. We found that rhythmic transcription predominantly drives rhythmic mRNA accumulation and translation for a majority of genes. Comparison of wild-type and Bmal1 KO mice shows that circadian clock and feeding rhythms have broad impact on rhythmic gene expression, Bmal1 deletion affecting surprisingly both transcriptional and posttranscriptional levels. Translation efficiency is differentially regulated during the diurnal cycle for genes with 5'-Terminal Oligo Pyrimidine tract (5'-TOP) sequences and for genes involved in mitochondrial activity, many harboring a Translation Initiator of Short 5'-UTR (TISU) motif. The increased translation efficiency of 5'-TOP and TISU genes is mainly driven by feeding rhythms but Bmal1 deletion also affects amplitude and phase of translation, including TISU genes. Together this study emphasizes the complex interconnections between circadian and feeding rhythms at several steps ultimately determining rhythmic gene expression and translation.
Mots-clé
circadian rhythms, ribosome profiling, mRNA translation, 5 '-TOP sequences, TISU motifs
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/12/2015 17:29
Dernière modification de la notice
20/08/2019 16:30
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