In vitro characterization of PlyE146, a novel phage lysin that targets Gram-negative bacteria.

Détails

Ressource 1Télécharger: journal.pone.0192507.pdf (4679.34 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_FFBEE644C544
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vitro characterization of PlyE146, a novel phage lysin that targets Gram-negative bacteria.
Périodique
PloS one
Auteur(s)
Larpin Y., Oechslin F., Moreillon P., Resch G., Entenza J.M., Mancini S.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
13
Numéro
2
Pages
e0192507
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Résumé
The recent rise of multidrug-resistant Gram-negative bacteria represents a serious threat to public health and makes the search for novel effective alternatives to antibiotics a compelling need. Bacteriophage (Phage) lysins are enzymes that hydrolyze the cell wall of bacteria and represent a promising alternative to tackle this ever-increasing problem. Despite their use is believed to be restricted to Gram-positive bacteria, recent findings have shown that they can also be used against Gram-negative bacteria. By using a phage genome-based screening approach, we identified and characterized a novel lysin, PlyE146, encoded by an Escherichia coli prophage and with a predicted molecular mass of ca. 17 kDa. PlyE146 is composed of a C-terminal cationic peptide and a N-terminal N-acetylmuramidase domain. Histidine-tagged PlyE146 was overexpressed from a plasmid in Lactococcus lactis NZ9000 and purified by NI-NTA chromatography. PlyE146 exhibited in vitro optimal bactericidal activity against E. coli K12 (3.6 log10 CFU/mL decrease) after 2 h of incubation at 37°C at a concentration of 400 μg/mL in the absence of NaCl and at pH 6.0. Under these conditions, PlyE146 displayed antimicrobial activity towards several other E. coli, Pseudomonas aeruginosa (3 to 3.8-log10 CFU/mL decrease) and Acinetobacter baumannii (4.9 to >5-log10 CFU/mL decrease) strains. Therefore, PlyE146 represents a promising therapeutic agent against E. coli, P. aeruginosa and A. baumannii infections. However, further studies are required to improve the efficacy of PlyE146 under physiological conditions.

Mots-clé
Anti-Bacterial Agents/pharmacology, Blotting, Western, Coliphages/metabolism, Glycoside Hydrolases/metabolism, Gram-Negative Bacteria/drug effects, Gram-Negative Bacteria/metabolism, Microscopy, Electron, Transmission
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/02/2018 18:46
Dernière modification de la notice
09/05/2019 4:04
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