Microarray deacetylation maps determine genome-wide functions for yeast histone deacetylases.

Détails

ID Serval
serval:BIB_FF2E3F89F87B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Microarray deacetylation maps determine genome-wide functions for yeast histone deacetylases.
Périodique
Cell
Auteur(s)
Robyr D., Suka Y., Xenarios I., Kurdistani S.K., Wang A., Suka N., Grunstein M.
ISSN
0092-8674 (Print)
ISSN-L
0092-8674
Statut éditorial
Publié
Date de publication
2002
Volume
109
Numéro
4
Pages
437-446
Langue
anglais
Résumé
Yeast contains a family of five related histone deacetylases (HDACs) whose functions are known at few genes. Therefore, we used chromatin immunoprecipitation and intergenic microarrays to generate genome-wide HDAC enzyme activity maps. Rpd3 and Hda1 deacetylate mainly distinct promoters and gene classes where they are recruited largely by novel mechanisms. Hda1 also deacetylates subtelomeric domains containing normally repressed genes that are used instead for gluconeogenesis, growth on carbon sources other than glucose, and adverse growth conditions. These domains have certain features of heterochromatin but are distinct from subtelomeric heterochromatin repressed by the deacetylase Sir2. Finally, Hos1/Hos3 and Hos2 preferentially affect ribosomal DNA and ribosomal protein genes, respectively. Thus, acetylation microarrays uncover the "division of labor" for yeast histone deacetylases.
Mots-clé
Carrier Proteins/genetics, Carrier Proteins/metabolism, Chromosome Mapping, DNA/genetics, DNA/metabolism, DNA, Ribosomal/genetics, DNA, Ribosomal/metabolism, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Fungal Proteins/genetics, Fungal Proteins/metabolism, Gene Expression Regulation, Enzymologic/genetics, Gene Expression Regulation, Fungal/genetics, Genome, Fungal, Heterochromatin/genetics, Heterochromatin/metabolism, Histone Deacetylases/genetics, Histone Deacetylases/metabolism, Histones/genetics, Histones/metabolism, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, Oligonucleotide Array Sequence Analysis, Plant Proteins, Protein Structure, Tertiary/genetics, Repressor Proteins, Ribosomal Proteins/genetics, Ribosomal Proteins/metabolism, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Saccharomyces cerevisiae Proteins/genetics, Saccharomyces cerevisiae Proteins/metabolism, Schizosaccharomyces pombe Proteins, Transcription Factors
Pubmed
Web of science
Open Access
Oui
Création de la notice
18/10/2012 9:08
Dernière modification de la notice
20/08/2019 16:29
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