Article: article from journal or magazin.
Thymus-independent generation of NK1+ T cells in vitro from fetal liver precursors.
Journal of Immunology
NK1.1+TCR alpha beta+ (NK1+) T cells are an unusual subset of mouse TCR alpha beta+ cells found primarily in adult thymus and liver. In contrast to conventional TCR alpha beta+ cells, NK1+ T cells have a TCR repertoire that is highly skewed to V alpha14 and to Vbeta8, -7, and -2. The developmental origin and ligand specificity of NK1+ T cells are controversial. We show here that NK1+ T cells with a typically biased V alpha and V beta repertoire develop in cytokine-supplemented suspension cultures of fetal liver established from either normal or athymic mice. Furthermore, NK1+ T cell development in fetal liver cultures is abrogated in beta2m-deficient mice (which lack MHC class I and other related molecules) and can be partially inhibited by the presence of anti-CD1 mAbs. Moreover, mixing experiments indicate that recombination-deficient SCID fetal liver cells can reconstitute NK1+ T cell development in beta2m-deficient fetal liver cultures. Collectively, our data demonstrate that NK1+ T cells can develop extrathymically from fetal liver precursors and that a beta2m-associated ligand (putatively CD1) present on nonlymphoid cells is essential for their positive selection and/or expansion.
Animals, Antigens, CD1/immunology, Cell Differentiation, Cells, Cultured, Female, Hematopoietic Stem Cells/cytology, Hematopoietic Stem Cells/immunology, Killer Cells, Natural/cytology, Killer Cells, Natural/immunology, Liver/cytology, Liver/embryology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Pregnancy, Receptors, Antigen, T-Cell, alpha-beta/immunology, Thymus Gland/cytology, Thymus Gland/immunology
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