CFH exerts anti-oxidant effects on retinal pigment epithelial cells independently from protecting against membrane attack complex.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_FE40D5654A9B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CFH exerts anti-oxidant effects on retinal pigment epithelial cells independently from protecting against membrane attack complex.
Journal
Scientific reports
Author(s)
Borras C., Canonica J., Jorieux S., Abache T., El Sanharawi M., Klein C., Delaunay K., Jonet L., Salvodelli M., Naud M.C., Arsenijevic Y., Shalabi A., Souchaud L., Behar-Cohen F., Dinet V.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
25/09/2019
Peer-reviewed
Oui
Volume
9
Number
1
Pages
13873
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Age Related Macular Degeneration (AMD) is the first cause of social blindness in people aged over 65 leading to atrophy of retinal pigment epithelial cells (RPE), photoreceptors and choroids, eventually associated with choroidal neovascularization. Accumulation of undigested cellular debris within RPE cells or under the RPE (Drusen), oxidative stress and inflammatory mediators contribute to the RPE cell death. The major risk to develop AMD is the Y402H polymorphism of complement factor H (CFH). CFH interacting with oxidized phospholipids on the RPE membrane modulates the functions of these cells, but the exact role of CFH in RPE cell death and survival remain poorly understood. The aim of this study was to analyze the potential protective mechanism of CFH on RPE cells submitted to oxidative stress. Upon exposure to oxidized lipids 4-HNE (4-hydroxy-2-nonenal) derived from photoreceptors, both the human RPE cell line ARPE-19 and RPE cells derived from human induced pluripotent stem cells were protected from death only in the presence of the full length human recombinant CFH in the culture medium. This protective effect was independent from the membrane attack complex (MAC) formation. CFH maintained RPE cells tight junctions' structure and regulated the caspase dependent apoptosis process. These results demonstrated the CFH anti-oxidative stress functions independently of its capacity to inhibit MAC formation.
Pubmed
Web of science
Open Access
Yes
Create date
13/10/2019 17:18
Last modification date
15/01/2021 7:12
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