Clostridium difficile toxin-induced inflammation and intestinal injury are mediated by the inflammasome.

Details

Serval ID
serval:BIB_FE30F99DCED6
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Clostridium difficile toxin-induced inflammation and intestinal injury are mediated by the inflammasome.
Journal
Gastroenterology
Author(s)
Ng J., Hirota S.A., Gross O., Li Y., Ulke-Lemee A., Potentier M.S., Schenck L.P., Vilaysane A., Seamone M.E., Feng H., Armstrong G.D., Tschopp J., Macdonald J.A., Muruve D.A., Beck P.L.
ISSN
1528-0012[electronic], 0016-5085[linking]
Publication state
Published
Issued date
2010
Peer-reviewed
Oui
Volume
139
Number
2
Pages
542-552
Language
english
Abstract
BACKGROUND & AIMS: Clostridium difficile-associated disease (CDAD) is the leading cause of nosocomial diarrhea in the United States. C difficile toxins TcdA and TcdB breach the intestinal barrier and trigger mucosal inflammation and intestinal damage. The inflammasome is an intracellular danger sensor of the innate immune system. In the present study, we hypothesize that TcdA and TcdB trigger inflammasome-dependent interleukin (IL)-1beta production, which contributes to the pathogenesis of CDAD. METHODS: Macrophages exposed to TcdA and TcdB were assessed for IL-1beta production, an indication of inflammasome activation. Macrophages deficient in components of the inflammasome were also assessed. Truncated/mutated forms of TcdB were assessed for their ability to activate the inflammasome. The role of inflammasome signaling in vivo was assessed in ASC-deficient and IL-1 receptor antagonist-treated mice. RESULTS: TcdA and TcdB triggered inflammasome activation and IL-1beta secretion in macrophages and human mucosal biopsy specimens. Deletion of Nlrp3 decreased, whereas deletion of ASC completely abolished, toxin-induced IL-1beta release. TcdB-induced IL-1beta release required recognition of the full-length toxin but not its enzymatic function. In vivo, deletion of ASC significantly reduced toxin-induced inflammation and damage, an effect that was mimicked by pretreatment with the IL-1 receptor antagonist anakinra. CONCLUSIONS: TcdA and TcdB trigger IL-1beta release by activating an ASC-containing inflammasome, a response that contributes to toxin-induced inflammation and damage in vivo. Pretreating mice with the IL-1 receptor antagonist anakinra afforded the same level of protection that was observed in ASC-/- mice. These data suggest that targeting inflammasome or IL-1beta signaling may represent new therapeutic targets in the treatment of CDAD.
Keywords
Animals, Bacterial Proteins/genetics, Bacterial Toxins/genetics, Biopsy, Carrier Proteins/genetics, Carrier Proteins/metabolism, Caspase 1/metabolism, Cell Line, Clostridium difficile/genetics, Clostridium difficile/pathogenicity, Cytoskeletal Proteins/deficiency, Cytoskeletal Proteins/genetics, Disease Models, Animal, Endocytosis, Endosomes/immunology, Endosomes/microbiology, Enterotoxins/genetics, Humans, Ileitis/immunology, Ileitis/microbiology, Ileum/drug effects, Ileum/immunology, Immunity, Innate/drug effects, Inflammation Mediators/metabolism, Interleukin 1 Receptor Antagonist Protein/pharmacology, Interleukin-1beta/metabolism, Macrophages/drug effects, Macrophages/immunology, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Mutation
Pubmed
Web of science
Create date
07/09/2010 16:27
Last modification date
20/08/2019 17:28
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