High dietary fat intake induces a microbiota signature that promotes food allergy.

Détails

ID Serval
serval:BIB_FDCCEC65868D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
High dietary fat intake induces a microbiota signature that promotes food allergy.
Périodique
The Journal of allergy and clinical immunology
Auteur(s)
Hussain M., Bonilla-Rosso G., Kwong Chung CKC, Bäriswyl L., Rodriguez M.P., Kim B.S., Engel P., Noti M.
ISSN
1097-6825 (Electronic)
ISSN-L
0091-6749
Statut éditorial
Publié
Date de publication
07/2019
Peer-reviewed
Oui
Volume
144
Numéro
1
Pages
157-170.e8
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Diet-induced obesity and food allergies increase in tandem, but a potential cause-and-effect relationship between these diseases of affluence remains to be tested.
We sought to test the role of high dietary fat intake, diet-induced obesity, and associated changes in gut microbial community structure on food allergy pathogenesis.
Mice were fed a high-fat diet (HFD) for 12 weeks before food allergen sensitization on an atopic dermatitis-like skin lesion, followed by intragastric allergen challenge to induce experimental food allergy. Germ-free animals were colonized with a signature HFD or lean microbiota for 8 weeks before induction of food allergy. Food-induced allergic responses were quantified by using a clinical allergy score, serum IgE levels, serum mouse mast cell protease 1 concentrations, and type 2 cytokine responses. Accumulation of intestinal mast cells was examined by using flow cytometry and chloroacetate esterase tissue staining. Changes in the gut microbial community structure were assessed by using high-throughput 16S ribosomal DNA gene sequencing.
HFD-induced obesity potentiates food-induced allergic responses associated with dysregulated intestinal effector mast cell responses, increased intestinal permeability, and gut dysbiosis. An HFD-associated microbiome was transmissible to germ-free mice, with the gut microbial community structure of recipients segregating according to the microbiota input source. Independent of an obese state, an HFD-associated gut microbiome was sufficient to confer enhanced susceptibility to food allergy.
These findings identify HFD-induced microbial alterations as risk factors for experimental food allergy and uncouple a pathogenic role of an HFD-associated microbiome from obesity. Postdieting microbiome alterations caused by overindulgence of dietary fat might increase susceptibility to food allergy.
Mots-clé
Food allergy, IgE, basophils, diet-induced obesity, dysbiosis, germ-free, high-fat diet, mast cells, microbiota
Pubmed
Web of science
Création de la notice
31/03/2019 14:26
Dernière modification de la notice
21/08/2019 5:34
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