Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry.
Details
Serval ID
serval:BIB_FD8BDFD69970
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Molecular identification of Mucorales in human tissues: contribution of PCR electrospray-ionization mass spectrometry.
Journal
Clinical microbiology and infection
Working group(s)
French Mycosis Study Group
Contributor(s)
Maizel J., Chouaki T., Hunault M., Bouchara J.P., Sirodot M., Gaillat J., Blanc X., Gobert Q., Leroy J., Legrand F., Navallou J., Grenouillet F., Million L., Blasco G., Malvy D., Accoceberry I., Ansart S., Moalic E., Reman O., Verdon R., Bonhomme J., Caillot D., Charles P., Chavanet P., Dalle F., Stahl J., Epaulard O., Thiebaut-Bertrand A., Cornet M., Guery B., Sendid B., Ferry T., Baudry T., Michallet M., Moutran M., Miailhes P., Bienvenue A., Faucher C., Gayet S., Ranque S., Jonquet O., Moreau P., Morio F., Roger P., Dellamonica P., Gari-Toussaint M., Veinstein A., Saulnier J., Kauffmann C., Arvieux C., Bernard M., Gandemer V., Gangneux J.P., Joly C., Pfister G., Veber B., Favennec L., Cazorla C., Raberin H., Alvarez M., Huynh A., Cassaing S., Giroux E., Dunand J., Naccache J., Gardin C., Freynet O., Brun S., Azoulay E., Lacroix C., Raffoux E., Joly V., Chochillon C., Charpentier J., Paugam A., Rivaud E., Cahen P., Novara A., Danaoui E., Cordonnier C., Botterel F., Bretagne S., Anguel N., Angoulvant A., Suarez F., Lacaille F., Oualha M., Bougnoux M., Combes A., Buffet P., Clair B., Dunand J., Maury E., Hennequin C., Tabone M., Wyplosz B., Merad M., Mihaila L.
ISSN
1469-0691 (Electronic)
ISSN-L
1198-743X
Publication state
Published
Issued date
06/2015
Peer-reviewed
Oui
Volume
21
Number
6
Pages
594.e1-5
Language
english
Notes
Publication types: Comparative Study ; Evaluation Study ; Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Molecular methods are crucial for mucormycosis diagnosis because cultures are frequently negative, even if microscopy suggests the presence of hyphae in tissues. We assessed PCR/electrospray-ionization mass spectrometry (PCR/ESI-MS) for Mucorales identification in 19 unfixed tissue samples from 13 patients with proven or probable mucormycosis and compared the results with culture, quantitative real-time PCR, 16S-23S rRNA gene internal transcribed spacer region (ITS PCR) and 18S PCR sequencing. Concordance with culture identification to both genus and species levels was higher for PCR/ESI-MS than for the other techniques. Thus, PCR/ESI-MS is suitable for Mucorales identification, within 6 hours, for tissue samples for which microscopy results suggest the presence of hyphae.
Keywords
DNA, Fungal/chemistry, DNA, Fungal/genetics, Humans, Microbiological Techniques/methods, Mucorales/genetics, Mucorales/isolation & purification, Mucormycosis/diagnosis, Pathology, Molecular/methods, Polymerase Chain Reaction/methods, Prospective Studies, Real-Time Polymerase Chain Reaction/methods, Sequence Analysis, DNA/methods, Spectrometry, Mass, Electrospray Ionization/methods, Time Factors, Culture, Mucorales identification, PCR, electrospray-ionization mass spectrometry, mucormycosis, species level
Pubmed
Web of science
Open Access
Yes
Create date
07/07/2023 11:04
Last modification date
18/07/2023 6:57
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