Cutting edge: alum adjuvant stimulates inflammatory dendritic cells through activation of the NALP3 inflammasome.

Details

Serval ID
serval:BIB_FD2879E86E02
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cutting edge: alum adjuvant stimulates inflammatory dendritic cells through activation of the NALP3 inflammasome.
Journal
Journal of Immunology
Author(s)
Kool M., Pétrilli V., De Smedt T., Rolaz A., Hammad H., van Nimwegen M., Bergen I.M., Castillo R., Lambrecht B.N., Tschopp J.
ISSN
1550-6606[electronic]
Publication state
Published
Issued date
2008
Volume
181
Number
6
Pages
3755-3759
Language
english
Abstract
Adjuvants are vaccine additives that stimulate the immune system without having any specific antigenic effect of itself. In this study we show that alum adjuvant induces the release of IL-1beta from macrophages and dendritic cells and that this is abrogated in cells lacking various NALP3 inflammasome components. The NALP3 inflammasome is also required in vivo for the innate immune response to OVA in alum. The early production of IL-1beta and the influx of inflammatory cells into the peritoneal cavity is strongly reduced in NALP3-deficient mice. The activation of adaptive cellular immunity to OVA-alum is initiated by monocytic dendritic cell precursors that induce the expansion of Ag-specific T cells in a NALP3-dependent way. We propose that, in addition to TLR stimulators, agonists of the NALP3 inflammasome should also be considered as vaccine adjuvants.
Keywords
Adjuvants, Immunologic/pharmacology, Alum Compounds/pharmacology, Aluminum Hydroxide/pharmacology, Animals, Carrier Proteins/biosynthesis, Carrier Proteins/genetics, Dendritic Cells/immunology, Dendritic Cells/metabolism, Female, Immunologic Factors/pharmacology, Inflammation Mediators/pharmacology, Macrophage Activation/immunology, Macrophages, Peritoneal/immunology, Macrophages, Peritoneal/metabolism, Magnesium Hydroxide/pharmacology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout
Pubmed
Web of science
Create date
30/10/2009 12:31
Last modification date
20/08/2019 16:28
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