Connexin 43 expression in human hypertrophied heart due to pressure and volume overload.

Détails

ID Serval
serval:BIB_FC61C3C4AD31
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Connexin 43 expression in human hypertrophied heart due to pressure and volume overload.
Périodique
Physiological Research / Academia Scientiarum Bohemoslovaca
Auteur(s)
Vetter C., Zweifel M., Zuppinger C., Carrel T., Martin D., Haefliger J.A., Delacrétaz E.
ISSN
0862-8408[print], 0862-8408[linking]
Statut éditorial
Publié
Date de publication
2010
Peer-reviewed
Oui
Volume
59
Numéro
1
Pages
35-42
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Résumé
Left ventricular hypertrophy (LVH) is due to pressure overload or mechanical stretch and is thought to be associated with remodeling of gap-junctions. We investigated whether the expression of connexin 43 (Cx43) is altered in humans in response to different degrees of LVH. The expression of Cx43 was analyzed by quantitative polymerase chain reaction, Western blot analysis and immunohistochemistry on left ventricular biopsies from patients undergoing aortic or mitral valve replacement. Three groups were analyzed: patients with aortic stenosis with severe LVH (n=9) versus only mild LVH (n=7), and patients with LVH caused by mitral regurgitation (n=5). Cx43 mRNA expression and protein expression were similar in the three groups studied. Furthermore, immunohistochemistry revealed no change in Cx43 distribution. We can conclude that when compared with mild LVH or with LVH due to volume overload, severe LVH due to chronic pressure overload is not accompanied by detectable changes of Cx43 expression or spatial distribution.
Mots-clé
Aged, Aged, 80 and over, Aortic Valve Stenosis/complications, Aortic Valve Stenosis/metabolism, Biopsy, Blood Pressure, Blotting, Western, Connexin 43/analysis, Connexin 43/genetics, Female, Gene Expression Regulation, Humans, Hypertrophy, Left Ventricular/etiology, Hypertrophy, Left Ventricular/mortality, Immunohistochemistry, Male, Middle Aged, Mitral Valve Insufficiency/complications, Mitral Valve Insufficiency/metabolism, Myocardium/chemistry, Myocardium/pathology, RNA, Messenger/analysis, Reverse Transcriptase Polymerase Chain Reaction, Severity of Illness Index, Ventricular Function, Left
Pubmed
Web of science
Création de la notice
11/03/2011 13:07
Dernière modification de la notice
20/08/2019 17:27
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