A novel cell-based sensor detecting the activity of individual basic proprotein convertases.

Détails

ID Serval
serval:BIB_FB3BEDCEB392
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A novel cell-based sensor detecting the activity of individual basic proprotein convertases.
Périodique
The FEBS journal
Auteur(s)
Löw K., Hardes K., Fedeli C., Seidah N.G., Constam D.B., Pasquato A., Steinmetzer T., Roulin A., Kunz S.
ISSN
1742-4658 (Electronic)
ISSN-L
1742-464X
Statut éditorial
In Press
Peer-reviewed
Oui
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: aheadofprint
Résumé
The basic proprotein convertases (PCs) furin, PC1/3, PC2, PC5/6, PACE4, PC4, and PC7 are promising drug targets for human diseases. However, developing selective inhibitors remains challenging due to overlapping substrate recognition motifs and limited structural information. Classical drug screening approaches for basic PC inhibitors involve homogeneous biochemical assays using soluble recombinant enzymes combined with fluorogenic substrate peptides that may not accurately recapitulate the complex cellular context of the basic PC-substrate interaction. Herein we report basic PC sensor (BPCS), a novel cell-based molecular sensor that allows rapid screening of candidate inhibitors and their selectivity toward individual basic PCs within mammalian cells. BPCS consists of Gaussia luciferase linked to a sortilin-1 membrane anchor via a cleavage motif that allows efficient release of luciferase specifically if individual basic PCs are provided in the same membrane. Screening of selected candidate peptidomimetic inhibitors revealed that BPCS can readily distinguish between general and selective PC inhibitors in a high-throughput screening format. The robust and cost-effective assay format of BPCS makes it suitable to identify novel specific small molecule inhibitors against basic PCs for therapeutic application. Its cell-based nature will allow screening for drug targets in addition to the catalytically active mature enzyme, including maturation, transport, and cellular factors that modulate the enzyme's activity. This broadened "target range" will enhance the likelihood to identify novel small molecule compounds that inhibit basic PCs in a direct or indirect manner and represents a conceptual advantage. This article is protected by copyright. All rights reserved.
Mots-clé
Protease, furin, high-throughput screening, inhibitor, proprotein convertase, sensor
Pubmed
Création de la notice
21/07/2019 16:53
Dernière modification de la notice
21/08/2019 6:32
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