Genomic organization of the human T-cell receptor variable alpha (TCRAV) gene cluster
Details
Serval ID
serval:BIB_FB15E1D4F73B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genomic organization of the human T-cell receptor variable alpha (TCRAV) gene cluster
Journal
Genomics
ISSN
0888-7543 (Print)
Publication state
Published
Issued date
07/1995
Volume
28
Number
2
Pages
131-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 20
Research Support, Non-U.S. Gov't --- Old month value: Jul 20
Abstract
A long-range physical map of the human T-cell receptor variable alpha (TCRAV) locus was produced using 23 V alpha subgroup-specific probes. Linkage disequilibrium across the locus was also studied using polymorphic TCRAV markers. Pulsed-field gel electrophoresis was used to map V alpha gene segments onto one SfiI fragment of 500 kb and two of 200 kb using DNA from peripheral blood neutrophils. PCR and conventional Southern techniques on Jurkat, CEM, and H9 T-cell lines were used to establish the 5' to 3' order of the gene segments and the relative positions of V alpha gene segments on the SfiI fragments. The linkage disequilibrium study used single-stranded conformation polymorphism analysis to genotype 100 normal caucasoid subjects for TCRAV5S1, V6S1, V8S1, V17S1, and V21S1 polymorphisms. Strong linkage disequilibrium was detected between V5S1 and V8S1, in concordance with the physical map. This new information will be useful for future studies of genetic variation at the TCRAV locus, its role in the shaping of the TCR repertoire, and its possible contribution to autoimmune diseases.
Keywords
Base Sequence
Chromosome Mapping
*Chromosomes, Human, Pair 14
DNA Probes
Electrophoresis, Gel, Pulsed-Field
European Continental Ancestry Group/genetics
*Genes
Humans
Linkage Disequilibrium
Molecular Sequence Data
*Multigene Family
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Single-Stranded Conformational
Receptors, Antigen, T-Cell, alpha-beta/*genetics
Sequence Deletion
T-Lymphocytes
Tumor Cells, Cultured
Pubmed
Web of science
Create date
25/01/2008 9:39
Last modification date
20/08/2019 17:26