Lentivirus-mediated expression of glutathione peroxidase: neuroprotection in murine models of Parkinson's disease

Détails

ID Serval
serval:BIB_FAC2B0105D0C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Lentivirus-mediated expression of glutathione peroxidase: neuroprotection in murine models of Parkinson's disease
Périodique
Neurobiology of Disease
Auteur(s)
Ridet  J. L., Bensadoun  J. C., Deglon  N., Aebischer  P., Zurn  A. D.
ISSN
0969-9961 (Print)
Statut éditorial
Publié
Date de publication
01/2006
Volume
21
Numéro
1
Pages
29-34
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Résumé
Reactive oxygen species are considered to contribute to the pathogenesis of Parkinson's disease (PD). In order to study viral vector-mediated overexpression of the antioxidant enzyme glutathione peroxidase (GPX) as a potential neuroprotective approach in both an in vitro and in vivo model of PD, we have developed a lentiviral vector carrying the human GPX1 gene. Neuroblastoma cells infected with this vector showed a 2-fold increase in GPX activity compared to cells infected with a control vector. In addition, overexpression of GPX protected 83.0 +/- 14.2% of these cells against 6-hydroxydopamine (6-OHDA)-induced toxicity, while only 22.9 +/- 4.6% of the cells infected with a control vector survived. Furthermore, lentivirus-mediated expression of GPX1 in nigral dopaminergic neurons in vivo prior to intrastriatal injection of 6-OHDA led to a small, but significant protection of these cells against drug-induced toxicity. These results indicate that antioxidative gene therapy strategies may be relevant for PD.
Mots-clé
Animals Antioxidants/metabolism Cell Line, Tumor Disease Models, Animal *Gene Expression Regulation, Enzymologic Gene Therapy/*methods Glutathione Peroxidase/*genetics Lentivirus/*genetics Male Mice Mice, Inbred C57BL Mice, Transgenic Neuroblastoma Oxidopamine/toxicity Parkinson Disease/genetics/metabolism/*therapy Sympatholytics/toxicity
Pubmed
Web of science
Création de la notice
28/01/2008 9:44
Dernière modification de la notice
03/03/2018 22:56
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