Glucose transporter 2 mediates the hypoglycemia-induced increase in cerebral blood flow.

Détails

ID Serval
serval:BIB_FAB7A3D6BC78
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Glucose transporter 2 mediates the hypoglycemia-induced increase in cerebral blood flow.
Périodique
Journal of cerebral blood flow and metabolism
Auteur(s)
Lei H., Preitner F., Labouèbe G., Gruetter R., Thorens B.
ISSN
1559-7016 (Electronic)
ISSN-L
0271-678X
Statut éditorial
Publié
Date de publication
09/2019
Peer-reviewed
Oui
Volume
39
Numéro
9
Pages
1725-1736
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Glucose transporter 2 (Glut2)-positive cells are sparsely distributed in brain and play an important role in the stimulation of glucagon secretion in response to hypoglycemia. We aimed to determine if Glut2-positive cells can influence another response to hypoglycemia, i.e. increased cerebral blood flow (CBF). CBF of adult male mice devoid of Glut2, either globally (ripglut1:glut2 <sup>-</sup> <sup>/</sup> <sup>-</sup> ) or in the nervous system only (NG2KO), and their respective controls were studied under basal glycemia and insulin-induced hypoglycemia using quantitative perfusion magnetic resonance imaging at 9.4 T. The effect on CBF of optogenetic activation of hypoglycemia responsive Glut2-positive neurons of the paraventricular thalamic area was measured in mice expressing channelrhodopsin2 under the control of the Glut2 promoter. We found that in both ripglut1:glut2 <sup>-</sup> <sup>/</sup> <sup>-</sup> mice and NG2KO mice, CBF in basal conditions was higher than in their respective controls and not further activated by hypoglycemia, as measured in the hippocampus, hypothalamus and whole brain. Conversely, optogenetic activation of Glut2-positive cells in the paraventricular thalamic nucleus induced a local increase in CBF similar to that induced by hypoglycemia. Thus, Glut2 expression in the nervous system is required for the control of CBF in response to changes in blood glucose concentrations.
Mots-clé
Glut2, cerebral blood flow, glut2 brain cells, hypoglycemia, optogenetic
Pubmed
Web of science
Création de la notice
22/03/2018 17:36
Dernière modification de la notice
01/10/2019 5:09
Données d'usage