Cell-Cell Contact Area Affects Notch Signaling and Notch-Dependent Patterning.

Détails

Ressource 1Télécharger: 28292428_BIB_FA8DF526122D.pdf (1965.14 [Ko])
Etat: Serval
Version: Author's accepted manuscript
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_FA8DF526122D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cell-Cell Contact Area Affects Notch Signaling and Notch-Dependent Patterning.
Périodique
Developmental cell
Auteur(s)
Shaya O., Binshtok U., Hersch M., Rivkin D., Weinreb S., Amir-Zilberstein L., Khamaisi B., Oppenheim O., Desai R.A., Goodyear R.J., Richardson G.P., Chen C.S., Sprinzak D.
ISSN
1878-1551 (Electronic)
ISSN-L
1534-5807
Statut éditorial
Publié
Date de publication
13/03/2017
Peer-reviewed
Oui
Volume
40
Numéro
5
Pages
505-511.e6
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
During development, cells undergo dramatic changes in their morphology. By affecting contact geometry, these morphological changes could influence cellular communication. However, it has remained unclear whether and how signaling depends on contact geometry. This question is particularly relevant for Notch signaling, which coordinates neighboring cell fates through direct cell-cell signaling. Using micropatterning with a receptor trans-endocytosis assay, we show that signaling between pairs of cells correlates with their contact area. This relationship extends across contact diameters ranging from micrometers to tens of micrometers. Mathematical modeling predicts that dependence of signaling on contact area can bias cellular differentiation in Notch-mediated lateral inhibition processes, such that smaller cells are more likely to differentiate into signal-producing cells. Consistent with this prediction, analysis of developing chick inner ear revealed that ligand-producing hair cell precursors have smaller apical footprints than non-hair cells. Together, these results highlight the influence of cell morphology on fate determination processes.

Mots-clé
Animals, Body Patterning, CHO Cells, Cell Communication, Chickens, Cricetinae, Cricetulus, Dogs, Endocytosis, Female, Humans, Madin Darby Canine Kidney Cells, Receptors, Notch/metabolism, Signal Transduction, Notch signaling, cell morphology, cell-cell contact, inner ear, lateral inhibition, live cell imaging
Pubmed
Web of science
Création de la notice
21/03/2017 18:52
Dernière modification de la notice
29/05/2019 7:10
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