The effects of glutamate and GABA (gamma-amino-butyric-acid) on spontaneous acetylcholine release at the neuromuscular junction in Xenopus laevis embryo cell cultures.

Détails

ID Serval
serval:BIB_FA85D3AEFEC5
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The effects of glutamate and GABA (gamma-amino-butyric-acid) on spontaneous acetylcholine release at the neuromuscular junction in Xenopus laevis embryo cell cultures.
Périodique
Science In China. Series B, Chemistry, Life Sciences and Earth Sciences
Auteur(s)
Xie Z.P., Tang Y.G., Stoop R., Mao J., Zhao N.M.,  G.N., Yang S.R., He X.P., Wang H., Liu C.G.
ISSN
1001-652X (Print)
ISSN-L
1001-652X
Statut éditorial
Publié
Date de publication
1993
Peer-reviewed
Oui
Volume
36
Numéro
9
Pages
1110-1116
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
The miniature endplate currents (MEPC's) were recorded at the neuromuscular junction of Xenopus laevis embryo neuron-muscle co-cultured cells. These MEPC's were due to the spontaneous release of acetylcholine from the nerve terminal. After perfusion with glutamate (10 mumol/L), both frequency and amplitude of the MEPC's increased. After washing away of glutamate, this effect persisted. We named this phenomena "Long-Term Facilitation". GABA (20 mumol/L) on the other hand had an inhibitory effect on both frequency and amplitude of the MEPC's. After washing away of GABA, the MEPC frequency and amplitude increased. We named this effect "Post-Potentiation". Local perfusion experiments furthermore indicated that the effect of glutamate was restricted to the neuromuscular junction, the effect of GABA was restricted to the soma.
Mots-clé
Acetylcholine/metabolism, Action Potentials/drug effects, Animals, Cells, Cultured, Glutamates/pharmacology, Membrane Potentials/drug effects, Motor Endplate/physiology, Neuromuscular Junction/metabolism, Xenopus laevis/embryology, gamma-Aminobutyric Acid/pharmacology
Pubmed
Création de la notice
29/10/2013 10:55
Dernière modification de la notice
20/08/2019 16:26
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