The effects of glutamate and GABA (gamma-amino-butyric-acid) on spontaneous acetylcholine release at the neuromuscular junction in Xenopus laevis embryo cell cultures.
Details
Serval ID
serval:BIB_FA85D3AEFEC5
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The effects of glutamate and GABA (gamma-amino-butyric-acid) on spontaneous acetylcholine release at the neuromuscular junction in Xenopus laevis embryo cell cultures.
Journal
Science In China. Series B, Chemistry, Life Sciences and Earth Sciences
ISSN
1001-652X (Print)
ISSN-L
1001-652X
Publication state
Published
Issued date
1993
Peer-reviewed
Oui
Volume
36
Number
9
Pages
1110-1116
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
The miniature endplate currents (MEPC's) were recorded at the neuromuscular junction of Xenopus laevis embryo neuron-muscle co-cultured cells. These MEPC's were due to the spontaneous release of acetylcholine from the nerve terminal. After perfusion with glutamate (10 mumol/L), both frequency and amplitude of the MEPC's increased. After washing away of glutamate, this effect persisted. We named this phenomena "Long-Term Facilitation". GABA (20 mumol/L) on the other hand had an inhibitory effect on both frequency and amplitude of the MEPC's. After washing away of GABA, the MEPC frequency and amplitude increased. We named this effect "Post-Potentiation". Local perfusion experiments furthermore indicated that the effect of glutamate was restricted to the neuromuscular junction, the effect of GABA was restricted to the soma.
Keywords
Acetylcholine/metabolism, Action Potentials/drug effects, Animals, Cells, Cultured, Glutamates/pharmacology, Membrane Potentials/drug effects, Motor Endplate/physiology, Neuromuscular Junction/metabolism, Xenopus laevis/embryology, gamma-Aminobutyric Acid/pharmacology
Pubmed
Create date
29/10/2013 11:55
Last modification date
20/08/2019 17:26
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