Central role for G protein-coupled phosphoinositide 3-kinase gamma in inflammation.

Détails

ID Serval
serval:BIB_FA6C7D42F7AD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Central role for G protein-coupled phosphoinositide 3-kinase gamma in inflammation.
Périodique
Science
Auteur(s)
Hirsch E., Katanaev V.L., Garlanda C., Azzolino O., Pirola L., Silengo L., Sozzani S., Mantovani A., Altruda F., Wymann M.P.
ISSN
0036-8075 (Print)
ISSN-L
0036-8075
Statut éditorial
Publié
Date de publication
2000
Volume
287
Numéro
5455
Pages
1049-1053
Langue
anglais
Résumé
Phosphoinositide 3-kinase (PI3K) activity is crucial for leukocyte function, but the roles of the four receptor-activated isoforms are unclear. Mice lacking heterotrimeric guanine nucleotide-binding protein (G protein)-coupled PI3Kgamma were viable and had fully differentiated neutrophils and macrophages. Chemoattractant-stimulated PI3Kgamma-/- neutrophils did not produce phosphatidylinositol 3,4,5-trisphosphate, did not activate protein kinase B, and displayed impaired respiratory burst and motility. Peritoneal PI3Kgamma-null macrophages showed a reduced migration toward a wide range of chemotactic stimuli and a severely defective accumulation in a septic peritonitis model. These results demonstrate that PI3Kgamma is a crucial signaling molecule required for macrophage accumulation in inflammation.
Mots-clé
Animals, Chemotactic Factors/pharmacology, Chemotaxis, Chemotaxis, Leukocyte/physiology, Enzyme Activation, Gene Targeting, Heterotrimeric GTP-Binding Proteins/metabolism, Isoenzymes/metabolism, Macrophages, Peritoneal/physiology, Mice, Mice, Inbred C57BL, Mice, Knockout, Neutrophils/metabolism, Neutrophils/physiology, Peritonitis/enzymology, Peritonitis/immunology, Phosphatidylinositol 3-Kinases/metabolism, Phosphatidylinositol Phosphates/metabolism, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins/metabolism, Proto-Oncogene Proteins c-akt, Respiratory Burst, Signal Transduction
Pubmed
Web of science
Création de la notice
09/07/2012 10:12
Dernière modification de la notice
03/03/2018 22:55
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