Glutamate reduces glucose utilization while concomitantly enhancing AQP9 and MCT2 expression in cultured rat hippocampal neurons.

Détails

Ressource 1Télécharger: BIB_FA36565FFEFB.P001.pdf (3867.54 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_FA36565FFEFB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Glutamate reduces glucose utilization while concomitantly enhancing AQP9 and MCT2 expression in cultured rat hippocampal neurons.
Périodique
Frontiers in Neuroscience
Auteur(s)
Tescarollo F., Covolan L., Pellerin L.
ISSN
1662-4548 (Print)
ISSN-L
1662-453X
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
8
Pages
246
Langue
anglais
Notes
pdf: Original Research ARTICLE
Résumé
The excitatory neurotransmitter glutamate has been reported to have a major impact on brain energy metabolism. Using primary cultures of rat hippocampal neurons, we observed that glutamate reduces glucose utilization in this cell type, suggesting alteration in mitochondrial oxidative metabolism. The aquaglyceroporin AQP9 and the monocarboxylate transporter MCT2, two transporters for oxidative energy substrates, appear to be present in mitochondria of these neurons. Moreover, they not only co-localize but they interact with each other as they were found to co-immunoprecipitate from hippocampal neuron homogenates. Exposure of cultured hippocampal neurons to glutamate 100 μM for 1 h led to enhanced expression of both AQP9 and MCT2 at the protein level without any significant change at the mRNA level. In parallel, a similar increase in the protein expression of LDHA was evidenced without an effect on the mRNA level. These data suggest that glutamate exerts an influence on neuronal energy metabolism likely through a regulation of the expression of some key mitochondrial proteins.
Pubmed
Web of science
Open Access
Oui
Création de la notice
09/12/2014 10:55
Dernière modification de la notice
09/05/2019 3:47
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