Cryo-electron microscopy of vitreous sections

Détails

ID Serval
serval:BIB_FA22CF179AB3
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Cryo-electron microscopy of vitreous sections
Périodique
EMBO Journal
Auteur(s)
Al-Amoudi  A., Chang  J. J., Leforestier  A., McDowall  A., Salamin  L. M., Norlen  L. P., Richter  K., Blanc  N. S., Studer  D., Dubochet  J.
ISSN
0261-4189 (Print)
Statut éditorial
Publié
Date de publication
09/2004
Volume
23
Numéro
18
Pages
3583-8
Notes
Journal Article
Review --- Old month value: Sep 15
Résumé
Since the beginning of the 1980s, cryo-electron microscopy of a thin film of vitrified aqueous suspension has made it possible to observe biological particles in their native state, in the absence of the usual artefacts of dehydration and staining. Combined with 3-d reconstruction, it has become an important tool for structural molecular biology. Larger objects such as cells and tissues cannot generally be squeezed in a thin enough film. Cryo-electron microscopy of vitreous sections (CEMOVIS) provides then a solution. It requires vitrification of a sizable piece of biological material and cutting it into ultrathin sections, which are observed in the vitrified state. Each of these operations raises serious difficulties that have now been overcome. In general, the native state seen with CEMOVIS is very different from what has been seen before and it is seen in more detail. CEMOVIS will give its full potential when combined with computerized electron tomography for 3-d reconstruction.
Mots-clé
Animals *Cryoelectron Microscopy Cyanobacteria/ultrastructure DNA/ultrastructure Desmosomes/ultrastructure Escherichia coli/ultrastructure Humans Pseudomonas aeruginosa/ultrastructure Skin/ultrastructure
Pubmed
Web of science
Création de la notice
24/01/2008 11:25
Dernière modification de la notice
03/03/2018 22:55
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