OATPB1/B3 and MRP3 expression in hepatocellular adenoma predicts Gd-EOB-DTPA uptake and correlates with risk of malignancy.

Détails

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Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_FA084F0A8BEA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
OATPB1/B3 and MRP3 expression in hepatocellular adenoma predicts Gd-EOB-DTPA uptake and correlates with risk of malignancy.
Périodique
Liver international
Auteur(s)
Sciarra A., Schmidt S., Pellegrinelli A., Maggioni M., Dondossola D., Pasquier J., Cigala C., Tosi D., Halkic N., Bulfamante G., Viale G., Bosari S., Balabaud C., Bioulac-Sage P., Sempoux C.
ISSN
1478-3231 (Electronic)
ISSN-L
1478-3223
Statut éditorial
Publié
Date de publication
01/2019
Peer-reviewed
Oui
Volume
39
Numéro
1
Pages
158-167
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Hepatobiliary phase (HBP) Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) has increased the accuracy in differentiating focal nodular hyperplasia (FNH) and hepatocellular adenoma (HCA). However, the ability of this technique to distinguish HCA subtypes remains controversial. The aim of this study was to investigate the expression of hepatocyte transporters (OATPB1/B3, MRP2, MRP3) in HCA subtypes, hence to understand their MRI signal intensity on HBP Gd-EOB-DTPA-enhanced MRI.
By means of immunohistochemistry (IHC), we scored the expression of OATPB1/B3, MRP2 and MRP3, in resected specimens of FNH (n = 40), subtyped HCA (n = 58) and HCA with focal malignant transformation (HCA-HCC, n = 4). Results were validated on a supplementary set of FNH (n = 6), subtyped HCA (n = 17) and HCA-HCC (n = 1) with Gd-EOB-DTPA MR images.
All FNH showed a preserved expression of hepatocytes transporters. Beta-catenin-activated HCA (at highest risk of malignant transformation) and HCA-HCC were characterized by preserved/increased OATPB1/B3 expression (predictor of hyperintensity on HBP), as opposed to other HCA subtypes (P < 0.01) that mostly showed OATPB1/B3 absence (predictor of hypointensity on HBP). HCA-HCC showed an additional MRP3 overexpressed profile (P < 0.01). On HBP Gd-EOB-DTPA-enhanced MRI, FNH and HCA signal intensity reflected the profile predicted by their specific OATPB1/B3 tissue expression. The hyperintense vs hypointense HBP signal criterion was able to distinguish all higher risk HCA and HCA-HCC (100% accuracy).
OATPB1/B3 and MRP3 IHC and signal intensity on HBP Gd-EOB-DTPA-enhanced MRI can help to stratify HCA according to their risk of malignant transformation.
Mots-clé
Hepatocellular adenoma, hepatocyte transporters, hepatospecific magnetic resonance imaging, radio-pathological correlation, hepatocellular adenoma
Pubmed
Web of science
Création de la notice
19/09/2018 15:53
Dernière modification de la notice
20/08/2019 17:25
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