Evaporation during preparation of unsupported thin vitrified aqueous layers for cryo-electron microscopy

Détails

ID Serval
serval:BIB_F9B2EA90C1A9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Evaporation during preparation of unsupported thin vitrified aqueous layers for cryo-electron microscopy
Périodique
Journal of Electron Microscopy Technique
Auteur(s)
Cyrklaff  M., Adrian  M., Dubochet  J.
ISSN
0741-0581 (Print)
Statut éditorial
Publié
Date de publication
12/1990
Volume
16
Numéro
4
Pages
351-5
Notes
Journal Article --- Old month value: Dec
Résumé
Evaporation of water cannot be fully avoided when an unsupported thin vitrified film of an aqueous suspension is prepared for cryo-electron microscopy. This results in increasing concentration of solute which could affect the observed material. We have quantitatively studied this effect by measuring the contrast of polystyrene spheres in a metrizamide solution. The drying effect is generally negligible when specimens are prepared on a hydrophilic perforated support but it is frequently important when hydrophobic films are used instead. A flow of humid air, double blotting with minimal exposure of the thin liquid film to the atmosphere, or an automatic plunger optimizing the blotting conditions are simple methods for reducing drying effects. With this third device acting on a hydrophilic supporting film, the increase of solute concentration is limited to less than 20%.
Mots-clé
Cold *Desiccation Humidity *Microscopy, Electron Microspheres Polystyrenes Reproducibility of Results
Pubmed
Web of science
Création de la notice
24/01/2008 11:25
Dernière modification de la notice
03/03/2018 22:54
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