Nanostructure of the epidermal extracellular space as observed by cryo-electron microscopy of vitreous sections of human skin.

Détails

ID Serval
serval:BIB_F99EE766A879
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Nanostructure of the epidermal extracellular space as observed by cryo-electron microscopy of vitreous sections of human skin.
Périodique
Journal of Investigative Dermatology
Auteur(s)
Al-Amoudi A., Dubochet J., Norlén L.
ISSN
0022-202X
Statut éditorial
Publié
Date de publication
2005
Peer-reviewed
Oui
Volume
124
Numéro
4
Pages
764-777
Langue
anglais
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Apr
Résumé
The newly developed method, cryo-electron microscopy of vitreous sections, was used to observe the nanostructure of the epidermal extracellular space. The data were obtained from vitreous sections of freshly taken, fully hydrated, non-cryo-protected human skin. The extracellular space of viable epidermis contains desmosomes, expressing a characteristic extracellular transverse approximately 5 nm periodicity, interconnected by a relatively electron lucent inter-desmosomal space. The extracellular space between viable and cornified epidermis contains transition desmosomes at different stages of reorganization interconnected by widened areas expressing a rich variety of complex membrane-like structures. The extracellular space of cornified epidermis contains approximately 9, approximately 14, approximately 25, approximately 33, approximately 39, approximately 44, and approximately 48 nm thick regions in turn containing one, two, four, six, eight, eight, and ten parallel electron-dense lines, respectively, between adjacent corneocyte lipid envelopes. The eight-line approximately 44 nm thick regions are most prevalent.
Mots-clé
Adult, Cell Differentiation, Cell Membrane, Cryoelectron Microscopy, Desmosomes, Epidermis, Extracellular Space, Humans, Lipid Bilayers, Male
Pubmed
Web of science
Création de la notice
24/01/2008 11:25
Dernière modification de la notice
03/03/2018 22:54
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