Distribution of muscarinic receptors on the endothelium of cortical vessels in the rat brain

Détails

ID Serval
serval:BIB_F99BC485A149
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Distribution of muscarinic receptors on the endothelium of cortical vessels in the rat brain
Périodique
Brain Research
Auteur(s)
Badaut  J., Moro  V., Seylaz  J., Lasbennes  F.
ISSN
0006-8993
Statut éditorial
Publié
Date de publication
12/1997
Peer-reviewed
Oui
Volume
778
Numéro
1
Pages
25-33
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec 5
Résumé
Functional and pharmacological studies have suggested that there are muscarinic receptors (mAChRs) on the endothelial cells of major cerebral arteries, while recent immunological studies indicate that there are no mAChRs on the endothelium of brain capillaries. This difference may be because the distribution of mAChR on the endothelium varies with the type of vessel. This paper examines the distribution of mAChR on the vascular endothelium along intraparenchymal blood vessels in the rat brain by immunolabelling and laser confocal microscopy. Sections were immunostained by combinations of an anti-mAChR antibody (M35) with antibodies to endothelial (anti-GLUT1), or to smooth muscle markers (anti-actin). Antibody labellings were detected with fluorescent second antibodies. Most of the penetrating vessels bore mAChR immunolabelling which coincided over almost all the vessel surface with endothelial labelling. The mAChR immunolabelling was less widespread over the endothelium on the medium sized vessels (diameter < 50 microm) and only 50% of these vessels had mAChR staining on the endothelium. There was no mAChR immunostaining on the endothelium of the capillaries. In contrast with the basilar artery, there was no mAChR immunolabelling on the smooth muscle layer of the intracortical vessels. These data indicate that the intensity of mAChR immunolabelling decreases along the vascular tree from large conducting vessels to capillaries.
Mots-clé
Actins/immunology Animals Cerebral Cortex/*blood supply Endothelium, Vascular/*chemistry Glucose Transporter Type 1 Image Processing, Computer-Assisted Immunohistochemistry Male Monosaccharide Transport Proteins Rats Rats, Wistar Receptors, Muscarinic/*analysis
Pubmed
Web of science
Création de la notice
29/01/2008 15:22
Dernière modification de la notice
03/03/2018 22:54
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