Expression of cholecystokinin in the duodenum of patients with coeliac disease: respective role of atrophy and lymphocytic infiltration.

Détails

ID Serval
serval:BIB_F99A09117EE1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Expression of cholecystokinin in the duodenum of patients with coeliac disease: respective role of atrophy and lymphocytic infiltration.
Périodique
Clinical Science (london, England : 1979)
Auteur(s)
Deprez P.H., Sempoux C., De Saeger C., Rahier J., Mainguet P., Pauwels S., Geubel A.
ISSN
0143-5221 (Print)
ISSN-L
0143-5221
Statut éditorial
Publié
Date de publication
2002
Peer-reviewed
Oui
Volume
103
Numéro
2
Pages
171-177
Langue
anglais
Notes
Publication types: Comparative Study ; Journal ArticlePublication Status: ppublish
Résumé
Cholecystokinin (CCK) release after a standard test meal is decreased in coeliac patients. The aim of the study was to determine the origin of the CCK deficiency and the relationship between the distinctive types of mucosal lesions observed in coeliac disease and the number of duodenal CCK cells and their peptide and mRNA content. Duodenal biopsies were obtained in ten controls and nineteen coeliac patients [seven with atrophic mucosa, six with increased numbers of intraepithelial lymphocytes (IELs) and six with fully normalized mucosa]. Immunocytochemistry was performed with a CCK C-terminal-specific polyclonal antiserum. The CCK cells were counted and related to the epithelial area using a semi-automated image analyser. CCK content in mucosal extracts was determined by radioimmunoassay. mRNA was measured with a semi-quantitative reverse transcriptase-PCR method using specific CCK and ribosomal protein L19 (RPL19) primers. CCK tissue concentration and CCK mRNA were significantly reduced in patients with atrophic mucosa [12.2 (range 6.9-17.5) pmol/g; CCK/RPL19 ratio 0.64 (0.30-0.99)] compared with patients with normal mucosa [40.5 (30.4-50.7) pmol/g; CCK/RPL19 ratio 1.40 (0.41-2.40)] or controls [42.7 (18.2-67.2) pmol/g; CCK/RPL19 ratio 1.35 (1.09-1.62)]. A similar decrease was observed in patients with an excess of IELs, 13.9 (3.8-31.8) pmol/g and 0.86 (0.57-1.15) pmol/g respectively. The number of CCK cells was, however, similar in all groups. Duodenal CCK concentration and mRNA are decreased not only in the mucosa presenting atrophic changes but also when disease activity is limited to infiltration by IELs. Reduced expression of the CCK gene could therefore be related to suppressive factors induced by the inflammatory infiltrate.
Mots-clé
Adult, Case-Control Studies, Celiac Disease/immunology, Celiac Disease/metabolism, Cell Count, Cholecystokinin/analysis, Cholecystokinin/genetics, Duodenum/chemistry, Duodenum/immunology, Female, Humans, Image Processing, Computer-Assisted, Intestinal Mucosa/chemistry, Intestinal Mucosa/immunology, Lymphocyte Count, Lymphocytes/immunology, Male, RNA, Messenger/analysis, Radioimmunoassay, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
20/10/2016 17:30
Dernière modification de la notice
03/03/2018 22:54
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