Replication origin of bacteriophage f1. Two signals required for its function.

Détails

ID Serval
serval:BIB_F981FC1F3503
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Replication origin of bacteriophage f1. Two signals required for its function.
Périodique
Journal of Molecular Biology
Auteur(s)
Dotto G.P., Horiuchi K., Jakes K.S., Zinder N.D.
ISSN
0022-2836 (Print)
ISSN-L
0022-2836
Statut éditorial
Publié
Date de publication
1982
Volume
162
Numéro
2
Pages
335-343
Langue
anglais
Résumé
Plus strand synthesis in bacteriophage f1 initiates in a region of dyad symmetry at a specific site (plus origin) recognized and nicked by the viral gene II protein. In this paper we describe several small deletions on the 5′ side of the f1 plus origin which disrupt the region of dyad symmetry and extend up to only four nucleotides from the gene II protein nicking site. These deletions do not impair the ability of gene II protein in vitro to nick this site. However, they do inhibit in vivo plus strand synthesis. We conclude that the nucleotide sequence at the f1 plus origin contains at least two specific signals that are required for efficient plus strand synthesis.
Mots-clé
Base Sequence, Coliphages/genetics, DNA Replication, DNA, Single-Stranded, DNA, Viral, Deoxyribonucleotides/analysis, Mutation, Viral Proteins/genetics, Virus Replication
Pubmed
Web of science
Création de la notice
24/01/2008 15:59
Dernière modification de la notice
03/03/2018 22:54
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