The CDK4-pRB-E2F1 pathway controls insulin secretion.

Détails

ID Serval
serval:BIB_F95F6A64CCD2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The CDK4-pRB-E2F1 pathway controls insulin secretion.
Périodique
Nature Cell Biology
Auteur(s)
Annicotte J.S., Blanchet E., Chavey C., Iankova I., Costes S., Assou S., Teyssier J., Dalle S., Sardet C., Fajas L.
ISSN
1476-4679 (Electronic)
ISSN-L
1465-7392
Statut éditorial
Publié
Date de publication
2009
Volume
11
Numéro
8
Pages
1017-1023
Langue
anglais
Résumé
CDK4-pRB-E2F1 cell-cycle regulators are robustly expressed in non-proliferating beta cells, suggesting that besides the control of beta-cell number the CDK4-pRB-E2F1 pathway has a role in beta-cell function. We show here that E2F1 directly regulates expression of Kir6.2, which is a key component of the K(ATP) channel involved in the regulation of glucose-induced insulin secretion. We demonstrate, through chromatin immunoprecipitation analysis from tissues, that Kir6.2 expression is regulated at the promoter level by the CDK4-pRB-E2F1 pathway. Consistently, inhibition of CDK4, or genetic inactivation of E2F1, results in decreased expression of Kir6.2, impaired insulin secretion and glucose intolerance in mice. Furthermore we show that rescue of Kir6.2 expression restores insulin secretion in E2f1(-/-) beta cells. Finally, we demonstrate that CDK4 is activated by glucose through the insulin pathway, ultimately resulting in E2F1 activation and, consequently, increased expression of Kir6.2. In summary we provide evidence that the CDK4-pRB-E2F1 regulatory pathway is involved in glucose homeostasis, defining a new link between cell proliferation and metabolism.
Mots-clé
Animals, Blotting, Western, COS Cells, Cell Line, Cercopithecus aethiops, Chromatin Immunoprecipitation, Cyclin-Dependent Kinase 4/metabolism, E2F1 Transcription Factor/genetics, E2F1 Transcription Factor/metabolism, Gene Expression Profiling, Glucose/pharmacology, Immunohistochemistry, Insulin/secretion, Islets of Langerhans/cytology, Islets of Langerhans/drug effects, Mice, Mice, Inbred C57BL, Mice, Knockout, Models, Biological, Phosphorylation, Potassium Channels, Inwardly Rectifying/genetics, Potassium Channels, Inwardly Rectifying/metabolism, Protein Binding, RNA, Small Interfering/genetics, Retinoblastoma Protein/metabolism, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction, Transfection
Pubmed
Web of science
Création de la notice
07/03/2013 17:00
Dernière modification de la notice
03/03/2018 22:53
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