Profiling treatment-specific post-translational modifications in a complex proteome with subtractive substrate phage display

Détails

ID Serval
serval:BIB_F95E06391CAD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Profiling treatment-specific post-translational modifications in a complex proteome with subtractive substrate phage display
Périodique
Proteomics
Auteur(s)
Tenzer  A., Hofstetter  B., Sauser  C., Bodis  S., Schubiger  A. P., Bonny  C., Pruschy  M.
ISSN
1615-9853 (Print)
Statut éditorial
Publié
Date de publication
09/2004
Volume
4
Numéro
9
Pages
2796-804
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
Proteolytic activation of zymogens or controlled degradation of inhibitory factors is part of a major regulatory system on the post-translational level to regulate treatment induced cellular stress responses. The identification of differential activity based substrates is thus of high interest to prioritize and validate candidate targets for drug discovery. Here we present a novel subtractive substrate phage display screening method for the selection of treatment induced post-translational peptide modifications in complex proteomes. We investigated this approach with tumor cells in response to a protease activating anticancer treatment modality using subtractive and iterative screening of cellular extracts derived from control and treated cells. Specific phage were identified that served as substrates for proteolytic activities in response to treatment related activity changes and could be distinguished from substrates for unspecific proteolytic background activities. Novel, selected peptide substrates were investigated in vitro and in vivo and showed high substrate specificity and functional biological significance.
Mots-clé
Amino Acid Sequence Animals Caspases/metabolism Cells, Cultured Enzyme-Linked Immunosorbent Assay/methods Humans Mice *Peptide Library Peptides/*chemistry/*metabolism *Protein Processing, Post-Translational Proteome/*analysis Reproducibility of Results
Pubmed
Web of science
Création de la notice
25/01/2008 15:16
Dernière modification de la notice
03/03/2018 22:53
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