P56LCK interacts via its src homology 2 domain with the ZAP-70 kinase.

Détails

ID Serval
serval:BIB_F924EABF77C1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
P56LCK interacts via its src homology 2 domain with the ZAP-70 kinase.
Périodique
Journal of Experimental Medicine
Auteur(s)
Duplay P., Thome M., Hervé F., Acuto O.
ISSN
0022-1007
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
179
Numéro
4
Pages
1163-1172
Langue
anglais
Résumé
p56lck, a member of the src family of protein tyrosine kinases, is an essential component in T cell receptor (TCR) signal transduction. p56lck contains a src homology 2 (SH2) domain found in a number of proteins involved in intracellular signaling. SH2 domains have been implicated in protein-protein interactions by binding to sequences in target proteins containing phosphorylated tyrosine. Using an in vitro assay, we have studied specific binding of tyrosine-phosphorylated proteins to a recombinant p56lck SH2 domain. In nonactivated Jurkat cells, two tyrosine-phosphorylated proteins were detected. Stimulation with anti-CD3 monoclonal antibodies induced the binding of seven additional tyrosine-phosphorylated proteins to the SH2 domain of p56lck. We have identified the zeta-associated tyrosine kinase, ZAP-70, as one of these proteins. Evidence suggests that binding of ZAP-70 to p56lck SH2 is direct and not mediated by zeta. The significance of this interaction was further investigated in vivo. p56lck could be coprecipitated with the zeta/ZAP-70 complex and conversely, ZAP-70 was detected in p56lck immunoprecipitates of activated Jurkat cells. The physical association of p56lck and ZAP-70 during activation supports the recently proposed functional cooperation of these two tyrosine kinases in TCR signaling.
Mots-clé
Antigens, CD3/immunology, Base Sequence, Cell Line, DNA, Humans, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Molecular Sequence Data, Protein-Tyrosine Kinases/metabolism, Receptors, Antigen, T-Cell/metabolism, Sequence Homology, Signal Transduction, ZAP-70 Protein-Tyrosine Kinase
Pubmed
Web of science
Création de la notice
24/01/2008 16:11
Dernière modification de la notice
03/03/2018 22:53
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