Derivation of traceable and transplantable photoreceptors from mouse embryonic stem cells.

Détails

Ressource 1Télécharger: BIB_F919AF069BA0.P001.pdf (4003.30 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_F919AF069BA0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Derivation of traceable and transplantable photoreceptors from mouse embryonic stem cells.
Périodique
Stem Cell Reports
Auteur(s)
Decembrini S., Koch U., Radtke F., Moulin A., Arsenijevic Y.
ISSN
2213-6711 (Electronic)
ISSN-L
2213-6711
Statut éditorial
Publié
Date de publication
2014
Peer-reviewed
Oui
Volume
2
Numéro
6
Pages
853-865
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: epublish
Résumé
Retinal degenerative diseases resulting in the loss of photoreceptors are one of the major causes of blindness. Photoreceptor replacement therapy is a promising treatment because the transplantation of retina-derived photoreceptors can be applied now to different murine retinopathies to restore visual function. To have an unlimited source of photoreceptors, we derived a transgenic embryonic stem cell (ESC) line in which the Crx-GFP transgene is expressed in photoreceptors and assessed the capacity of a 3D culture protocol to produce integration-competent photoreceptors. This culture system allows the production of a large number of photoreceptors recapitulating the in vivo development. After transplantation, integrated cells showed the typical morphology of mature rods bearing external segments and ribbon synapses. We conclude that a 3D protocol coupled with ESCs provides a safe and renewable source of photoreceptors displaying a development and transplantation competence comparable to photoreceptors from age-matched retinas.
Pubmed
Web of science
Open Access
Oui
Création de la notice
07/07/2014 8:48
Dernière modification de la notice
09/05/2019 3:43
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