Survey of urine from transplant recipients for polyomaviruses JC and BK using the polymerase chain reaction

Details

Serval ID
serval:BIB_F8FEF1DA6B47
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Survey of urine from transplant recipients for polyomaviruses JC and BK using the polymerase chain reaction
Journal
Molecular and Cellular Probes
Author(s)
Marshall  W. F., Telenti  A., Proper  J., Aksamit  A. J., Smith  T. F.
ISSN
0890-8508 (Print)
Publication state
Published
Issued date
04/1991
Volume
5
Number
2
Pages
125-8
Notes
Journal Article --- Old month value: Apr
Abstract
Using polymerase chain reaction (PCR), we examined 108 urine specimens from 39 post transplant patients for polyomaviruses JC (JCV) and BK (BKV). Urine sediments were collected and subjected to 30 cycles of amplification. PCR products were resolved by agarose gel electrophoresis, transferred to nylon membranes by Southern blot, and hybridized with radiolabelled probes. Polyomavirus DNA was found in urine specimens from 17 out of 39 patients (44%). Both viruses were detected in specimens from nine patients, JCV alone in five, and BKV alone in three. In comparison, polyomavirus was detected in only five of 22 PCR positive specimens by shell vial cell culture assay. Our results show a high prevalence of polyomavirus shedding after transplantation and suggest a higher rate of JC viruria than previously reported.
Keywords
DNA, Viral/*urine Heart Transplantation Humans Liver Transplantation Nucleic Acid Hybridization *Organ Transplantation *Polymerase Chain Reaction Polyomavirus/*genetics Tumor Virus Infections/microbiology
Pubmed
Web of science
Create date
25/01/2008 15:45
Last modification date
20/08/2019 17:24
Usage data