CD40-CD40 ligand interactions in vivo regulate migration of antigen-bearing dendritic cells from the skin to draining lymph nodes

Détails

ID Serval
serval:BIB_F8E9D8DFEB74
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
CD40-CD40 ligand interactions in vivo regulate migration of antigen-bearing dendritic cells from the skin to draining lymph nodes
Périodique
Journal of Experimental Medicine
Auteur(s)
Moodycliffe  A. M., Shreedhar  V., Ullrich  S. E., Walterscheid  J., Bucana  C., Kripke  M. L., Flores-Romo  L.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
06/2000
Volume
191
Numéro
11
Pages
2011-20
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun 5
Résumé
Whereas CD40-CD40 ligand interactions are important for various dendritic cell (DC) functions in vitro, their in vivo relevance is unknown. We analyzed the DC status of CD40 ligand -/- mice using a contact hypersensitivity (CHS) model system that enables multiple functions of DCs to be assessed in vivo. Immunohistochemistry of skin sections revealed no differences in terms of numbers and morphology of dendritic epidermal Langerhans cells (LCs) in unsensitized CD40 ligand -/- mice as compared with wild-type C57BL/6 mice. However, after contact sensitization of CD40 ligand -/- mice, LCs failed to migrate out of the skin and substantially fewer DCs accumulated in draining lymph nodes (DLNs). Furthermore, very few antigen-bearing DCs could be detected in the paracortical region of lymph nodes draining sensitized skin. This defect in DC migration after hapten sensitization was associated with defective CHS responses and decreased cutaneous tumor necrosis factor (TNF)-alpha production and was corrected by injecting recombinant TNF-alpha or an agonistic anti-CD40 monoclonal antibody. Thus, CD40-CD40 ligand interactions in vivo regulate the migration of antigen-bearing DCs from the skin to DLNs via TNF-alpha production and play a vital role in the initiation of acquired T cell-mediated immunity.
Mots-clé
Animals Antigens, CD40/*immunology CD40 Ligand Cell Count Cell Movement/*immunology Dermatitis, Contact Disease Models, Animal Langerhans Cells/classification/cytology/*immunology/physiology Lymph Nodes Membrane Glycoproteins/genetics/*immunology Mice Mice, Inbred C57BL Mice, Knockout Skin/cytology Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Création de la notice
25/01/2008 17:50
Dernière modification de la notice
03/03/2018 22:53
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