CD40-CD40 ligand interactions in vivo regulate migration of antigen-bearing dendritic cells from the skin to draining lymph nodes.
Details
Serval ID
serval:BIB_F8E9D8DFEB74
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CD40-CD40 ligand interactions in vivo regulate migration of antigen-bearing dendritic cells from the skin to draining lymph nodes.
Journal
The Journal of experimental medicine
ISSN
0022-1007
ISSN-L
0022-1007
Publication state
Published
Issued date
05/06/2000
Peer-reviewed
Oui
Volume
191
Number
11
Pages
2011-2020
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Publication Status: ppublish
Abstract
Whereas CD40-CD40 ligand interactions are important for various dendritic cell (DC) functions in vitro, their in vivo relevance is unknown. We analyzed the DC status of CD40 ligand -/- mice using a contact hypersensitivity (CHS) model system that enables multiple functions of DCs to be assessed in vivo. Immunohistochemistry of skin sections revealed no differences in terms of numbers and morphology of dendritic epidermal Langerhans cells (LCs) in unsensitized CD40 ligand -/- mice as compared with wild-type C57BL/6 mice. However, after contact sensitization of CD40 ligand -/- mice, LCs failed to migrate out of the skin and substantially fewer DCs accumulated in draining lymph nodes (DLNs). Furthermore, very few antigen-bearing DCs could be detected in the paracortical region of lymph nodes draining sensitized skin. This defect in DC migration after hapten sensitization was associated with defective CHS responses and decreased cutaneous tumor necrosis factor (TNF)-alpha production and was corrected by injecting recombinant TNF-alpha or an agonistic anti-CD40 monoclonal antibody. Thus, CD40-CD40 ligand interactions in vivo regulate the migration of antigen-bearing DCs from the skin to DLNs via TNF-alpha production and play a vital role in the initiation of acquired T cell-mediated immunity.
Keywords
Animals, CD40 Antigens/immunology, CD40 Ligand, Cell Count, Cell Movement/immunology, Dermatitis, Contact, Disease Models, Animal, Langerhans Cells/classification, Langerhans Cells/cytology, Langerhans Cells/immunology, Langerhans Cells/physiology, Lymph Nodes, Membrane Glycoproteins/genetics, Membrane Glycoproteins/immunology, Mice, Mice, Inbred C57BL, Mice, Knockout, Skin/cytology, Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:50
Last modification date
09/08/2024 11:37