Long synthetic peptides as biologically active proteins: the example of the chemokines.

Détails

ID Serval
serval:BIB_F8C31B6E07F2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Long synthetic peptides as biologically active proteins: the example of the chemokines.
Périodique
Biologicals : Journal of the International Association of Biological Standardization
Auteur(s)
Thierry A.C., Pinaud S., Bigler N., Perrenoud G., Denis B., Roggero M.A., Fasel N., Moulon C., Demotz S.
ISSN
1045-1056 (Print)
ISSN-L
1045-1056
Statut éditorial
Publié
Date de publication
2001
Volume
29
Numéro
3-4
Pages
259-263
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Chemokines constitute an expanding protein family of over 40 members which exhibit a wide variety of biological activities and are involved in many normal physiological processes, such as cellular migration, differentiation and activation, but also in pathological situations, such as inflammation and metastasis. Over the last few years, we have developed methods to manufacture long synthetic peptides of up to 130 residues, and to achieve the formation of native-like cysteine pairings. This ability prompted us to undertake the total chemical synthesis of chemokines. So far, we have successfully produced over 30 chemokine species, which exhibit biological activities similar to, or greater than, those reported by others. Chemical synthesis offers a clear advantage over recombinant technologies for the introduction of fluorochromes and haptens at molecularly defined positions. In addition, approval of chemically synthesized products for use in humans is straightforward compared with material produced by biological methods.
Mots-clé
Chemokines/chemistry, Chemokines/isolation & purification, Chromatography, High Pressure Liquid, Humans, Peptides/chemistry, Peptides/isolation & purification
Pubmed
Web of science
Création de la notice
24/01/2008 16:02
Dernière modification de la notice
03/03/2018 22:52
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