Profiling of proteolytic activities secreted by cancer cells using phage display substrate technology

Détails

ID Serval
serval:BIB_F86AA9544B21
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Profiling of proteolytic activities secreted by cancer cells using phage display substrate technology
Périodique
Tumour Biology
Auteur(s)
Cloutier  S. M., Kundig  C., Gygi  C. M., Jichlinski  P., Leisinger  H. J., Deperthes  D.
ISSN
1010-4283
Statut éditorial
Publié
Date de publication
04/2004
Peer-reviewed
Oui
Volume
25
Numéro
1-2
Pages
24-30
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan-Apr
Résumé
Although the cellular steps required for metastasis are similar for all cancer cells, proteases involved in this process and their expression levels vary greatly between different cancer types. Thus, the identification of these proteolytic activities represents a crucial issue in the understanding of cancer development. Until now, phage display substrate technology has been successfully employed for the characterization of purified proteases but was never used with a mix of proteases. In the present work, we report an easy protocol to identify multiple proteolytic activities secreted by cancer cells. We selected substrates from a phage display library of high diversity using secreted media of three established prostate cancer cell lines (DU-145, LNCaP and PC-3) with variable degrees of invasive capability. Some of these selected peptide substrates were hydrolyzed by the secreted proteins of all three prostatic cancer cell lines, demonstrating similarities in their proteolytic activities. On the other hand, a few substrates were cancer cell specific, indicating differences in the phenotypes of protease expression in prostate cancer. This work reports for the first time the selection of substrates from a mix of proteases using phage display technology and opens a new avenue for the direct identification of proteolytic activities for tumor extracts.
Mots-clé
Endopeptidases/isolation & purification/*pharmacology Humans Hydrolysis Male *Neoplasm Metastasis Neoplasms/*physiopathology Peptide Hydrolases/isolation & purification/*pharmacology *Peptide Library Prostatic Neoplasms/*enzymology Proteins/*metabolism Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
21/01/2008 17:10
Dernière modification de la notice
03/03/2018 22:52
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