Sialylation of the B lymphocyte molecule CD22 by alpha 2,6-sialyltransferase is implicated in the regulation of CD22-mediated adhesion.

Détails

ID Serval
serval:BIB_F82883D5CCDE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Sialylation of the B lymphocyte molecule CD22 by alpha 2,6-sialyltransferase is implicated in the regulation of CD22-mediated adhesion.
Périodique
Journal of Biological Chemistry
Auteur(s)
Braesch-Andersen S., Stamenkovic I.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
269
Numéro
16
Pages
11783-11786
Langue
anglais
Résumé
The B cell surface receptor CD22 binds several sialoglycoproteins containing sialic acid in alpha 2,6 linkage, on the surface of B and T lymphocytes. Because lymphocytes adhere tightly to fibroblasts transfected with CD22 cDNA, it would appear reasonable to suggest that regulatory mechanisms might have evolved which prevent undesired CD22-mediated leukocyte aggregation. Here we provide evidence for the existence of at least one mechanism that might regulate CD22 interaction with ligands on adjacent cells. We demonstrate that sialylation of CD22 by beta-galactoside alpha 2,6-sialyltransferase abrogates CD22-mediated lymphocyte adhesion, and that adhesion can be restored by removal of alpha 2,6-linked sialic acid residues from the CD22 molecule. Taken together, our results suggest that alpha 2,6-sialyltransferase can both promote and inhibit CD22-ligand interactions. These observations provide the first direct evidence that receptor-ligand interactions mediated by an Ig superfamily molecule are under the control of a specific glycosyltransferase.
Mots-clé
Animals, Antigens, CD/isolation & purification, Antigens, CD/metabolism, Antigens, CD22, Antigens, Differentiation, B-Lymphocyte/isolation & purification, Antigens, Differentiation, B-Lymphocyte/metabolism, B-Lymphocytes/immunology, B-Lymphocytes/metabolism, Burkitt Lymphoma, Cell Adhesion, Cell Adhesion Molecules/metabolism, Cell Line, Cercopithecus aethiops, Electrophoresis, Polyacrylamide Gel, Humans, Lectins, Leukemia, T-Cell, Molecular Weight, N-Acetylneuraminic Acid, Neuraminidase/metabolism, Recombinant Proteins/metabolism, Sialic Acids/metabolism, Sialyltransferases/metabolism, Transfection, Tumor Cells, Cultured
Pubmed
Web of science
Création de la notice
29/01/2008 19:34
Dernière modification de la notice
03/03/2018 22:51
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