Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance.

Détails

ID Serval
serval:BIB_F7DF71B2D2F2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Galactofuranose in Mycoplasma mycoides is important for membrane integrity and conceals adhesins but does not contribute to serum resistance.
Périodique
Molecular Microbiology
Auteur(s)
Schieck E., Lartigue C., Frey J., Vozza N., Hegermann J., Miller R.A., Valguarnera E., Muriuki C., Meens J., Nene V., Naessens J., Weber J., Lowary T.L., Vashee S., Feldman M.F., Jores J.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Statut éditorial
Publié
Date de publication
2016
Volume
99
Numéro
1
Pages
55-70
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Mycoplasma mycoides subsp. capri (Mmc) and subsp. mycoides (Mmm) are important ruminant pathogens worldwide causing diseases such as pleuropneumonia, mastitis and septicaemia. They express galactofuranose residues on their surface, but their role in pathogenesis has not yet been determined. The M. mycoides genomes contain up to several copies of the glf gene, which encodes an enzyme catalysing the last step in the synthesis of galactofuranose. We generated a deletion of the glf gene in a strain of Mmc using genome transplantation and tandem repeat endonuclease coupled cleavage (TREC) with yeast as an intermediary host for the genome editing. As expected, the resulting YCp1.1-Δglf strain did not produce the galactofuranose-containing glycans as shown by immunoblots and immuno-electronmicroscopy employing a galactofuranose specific monoclonal antibody. The mutant lacking galactofuranose exhibited a decreased growth rate and a significantly enhanced adhesion to small ruminant cells. The mutant was also 'leaking' as revealed by a β-galactosidase-based assay employing a membrane impermeable substrate. These findings indicate that galactofuranose-containing polysaccharides conceal adhesins and are important for membrane integrity. Unexpectedly, the mutant strain showed increased serum resistance.
Mots-clé
Adhesins, Bacterial/analysis, Animals, Bacterial Adhesion, Blood Bactericidal Activity, Cell Membrane/chemistry, Cell Membrane/physiology, Cells, Cultured, Disaccharides/analysis, Gene Deletion, Gene Targeting, Immunoblotting, Microscopy, Immunoelectron, Mycoplasma mycoides/chemistry, Mycoplasma mycoides/genetics, Sheep
Pubmed
Web of science
Création de la notice
03/12/2015 11:46
Dernière modification de la notice
03/03/2018 22:51
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