Modification of the monomer composition of polyhydroxyalkanoate synthesized in Saccharomyces cerevisiae expressing variants of the beta-oxidation-associated multifunctional enzyme.

Détails

ID Serval
serval:BIB_F7D26095E568
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Modification of the monomer composition of polyhydroxyalkanoate synthesized in Saccharomyces cerevisiae expressing variants of the beta-oxidation-associated multifunctional enzyme.
Périodique
Applied and Environmental Microbiology
Auteur(s)
Marchesini S., Erard N., Glumoff T., Hiltunen J.K., Poirier Y.
ISSN
0099-2240 (Print)
ISSN-L
0099-2240
Statut éditorial
Publié
Date de publication
2003
Volume
69
Numéro
11
Pages
6495-6499
Langue
anglais
Résumé
Expression by Saccharomyces cerevisiae of a polyhydroxyalkanoate (PHA) synthase modified at the carboxy end by the addition of a peroxisome targeting signal derived from the last 34 amino acids of the Brassica napus isocitrate lyase (ICL) and containing the terminal tripeptide Ser-Arg-Met resulted in the synthesis of PHA. The ability of the terminal peptide Ser-Arg-Met and of the 34-amino-acid peptide from the B. napus ICL to target foreign proteins to the peroxisome of S. cerevisiae was demonstrated with green fluorescent protein fusions. PHA synthesis was found to be dependent on the presence of both the enzymes generating the beta-oxidation intermediate 3-hydroxyacyl-coenzyme A (3-hydroxyacyl-[CoA]) and the peroxin-encoding PEX5 gene, demonstrating the requirement for a functional peroxisome and a beta-oxidation cycle for PHA synthesis. Using a variant of the S. cerevisiae beta-oxidation multifunctional enzyme with a mutation inactivating the B domain of the R-3-hydroxyacyl-CoA dehydrogenase, it was possible to modify the PHA monomer composition through an increase in the proportion of the short-chain monomers of five and six carbons.
Mots-clé
3-Hydroxyacyl CoA Dehydrogenases/genetics, Acyltransferases/genetics, Acyltransferases/metabolism, Enoyl-CoA Hydratase/genetics, Fatty Acids/metabolism, Gene Expression Regulation, Fungal, Genetic Engineering/methods, Multienzyme Complexes/genetics, Mutation, Oxidation-Reduction, Peroxisomes/enzymology, Polyesters/chemistry, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae/genetics
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 20:42
Dernière modification de la notice
09/05/2019 3:39
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