Exploration of the visual system: Part 1: Dissection of the mouse eye for RNA, protein, and histological analyses

Détails

ID Serval
serval:BIB_F7972540F109
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Exploration of the visual system: Part 1: Dissection of the mouse eye for RNA, protein, and histological analyses
Périodique
Current Protocols in Mouse Biology
Auteur(s)
Escher P., Schorderet D.F.
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
1
Pages
445‐462
Langue
anglais
Résumé
Due to the power of genetics, the mouse has become a widely used animal model in vision research. However, its eyeball has an axial length of only about 2 mm. The present protocol describes how to easily dissect the small rodent eye post mortem. This allows collecting different tissues of the eye, i.e., cornea, lens, iris, retina, optic nerve, retinal pigment epithelium (RPE), and sclera. We further describe in detail how to process these eye samples in order to obtain high‐quality RNA for RNA expression profiling studies. Depending on the eye tissue to be analyzed, we present appropriate lysis buffers to prepare total protein lysates for immunoblot and immuno‐precipitation analyses. Fixation, inclusion, embedding, and cryosectioning of the globe for routine histological analyses (HE staining, DAPI staining, immunohistochemistry, in situ hybridization) is further presented. These basic protocols should allow novice investigators to obtain eye tissue samples rapidly for their experiments.
Mots-clé
ophthalmology, visual sciences, retina, cornea, lens, iris, retinal pigment epithelium (RPE)
Création de la notice
24/01/2013 13:04
Dernière modification de la notice
19/03/2018 11:53
Données d'usage