Exploration of the visual system: Part 1: Dissection of the mouse eye for RNA, protein, and histological analyses

Details

Serval ID
serval:BIB_F7972540F109
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Exploration of the visual system: Part 1: Dissection of the mouse eye for RNA, protein, and histological analyses
Journal
Current Protocols in Mouse Biology
Author(s)
Escher P., Schorderet D.F.
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
1
Pages
445‐462
Language
english
Abstract
Due to the power of genetics, the mouse has become a widely used animal model in vision research. However, its eyeball has an axial length of only about 2 mm. The present protocol describes how to easily dissect the small rodent eye post mortem. This allows collecting different tissues of the eye, i.e., cornea, lens, iris, retina, optic nerve, retinal pigment epithelium (RPE), and sclera. We further describe in detail how to process these eye samples in order to obtain high‐quality RNA for RNA expression profiling studies. Depending on the eye tissue to be analyzed, we present appropriate lysis buffers to prepare total protein lysates for immunoblot and immuno‐precipitation analyses. Fixation, inclusion, embedding, and cryosectioning of the globe for routine histological analyses (HE staining, DAPI staining, immunohistochemistry, in situ hybridization) is further presented. These basic protocols should allow novice investigators to obtain eye tissue samples rapidly for their experiments.
Keywords
ophthalmology, visual sciences, retina, cornea, lens, iris, retinal pigment epithelium (RPE)
Create date
24/01/2013 12:04
Last modification date
20/08/2019 16:23
Usage data